6
Dry with a dry lens cleaning tissue.
7
Protect the flow cell from dust until you are ready to load it onto the instrument.
Load the Sequencing Flow Cell
1
Place the flow cell on the flow cell holder with the inlet and outlet ports facing
down
and the barcode on
the right. Make sure that the arrow on the left edge of the flow cell, which indicates flow direction, points
toward the instrument.
2
Gently slide the flow cell toward the top and right guide pins until it stops.
Figure 15
Flow Cell Positioned Against Top and Right Guide Pins
A
Top guide pin
B
Right guide pins
3
Remove your hand from the flow cell to prevent alignment drift over time.
4
Slowly move the flow cell lever to position 1 to engage the vacuum and secure the flow cell.
When the flow cell lever is blinking green, the vacuum is engaged. If the lever is not green, see
5
Wait about five seconds, and then slowly move the flow cell lever to position 2.
When the flow cell lever is solid green, the manifolds are in position and the flow cell is ready for use.
6
Make sure that the Vacuum Engaged checkbox is selected, and then select Next.
Confirm Proper Flow
Checking for proper flow confirms that the flow cell and gaskets are properly installed and the manifold is
engaged.
1
Select Position 5 from the drop-down list.
2
Confirm the following default values:
u
Volume: 250
u
Aspirate Rate: 250
u
Dispense Rate: 2000
3
Select Pump.
4
Inspect the flow cell for bubbles passing through the lanes or leaks near the manifolds.
5
If excessive bubbles are present, do as follows.
Document # 15011190 v03
For Research Use Only. Not for use in diagnostic procedures.
38
HiSeq 2000 System Guide