Figure 2
Flow Cell Stage With Two Flow Cells
A
Flow cell A
B
Flow cell B
C
Flow cell lever A
D
Flow cell lever B
Flow cell A is on the left, and flow cell B is on the right. Each flow cell is seated on the flow cell stage, which
moves in and out of the optics module as directed by the control software. The flow cell stage must be in the
forward-most position to open the flow cell compartment door and load or remove a flow cell.
The flow cell is positioned on the flow cell holder with the inlet and outlet ports facing down. A vacuum
beneath the flow cell holder holds the flow cell in place. The illuminated flow cell lever in front of each flow cell
holder controls the vacuum. The flow cell lever turns green when the vacuum seal is secure.
Reagent Compartment
The reagent compartment is a high-capacity reagent chiller that holds three reagent racks: two for
SBS reagents and 1 for indexing and paired-end reagents. Sipper handles lower the sippers into the reagent
bottles.
u
SBS reagent racks—Hold 250 ml conical bottles. The reagent rack for flow cell A is in the center position,
and the rack for flow cell B is in the far right position. Each reagent rack has numbered positions that
correspond to connections on an internal reagent selector valve.
u
Indexing and paired-end reagent rack—Located in the left position. It has two rows of numbered
positions that hold 15 ml conical tubes containing paired-end reagents and indexing reagents. The left
row is for flow cell A, and the right row is for flow cell B.
u
Reagent chiller—The reagent chiller houses the reagent racks and maintains an internal temperature of
2°C to 8°C.
Document # 15011190 v03
For Research Use Only. Not for use in diagnostic procedures.
3
HiSeq 2000 System Guide