ibidi Heating System, Multi-Well Plates, K-Frame
5.4
Temperature Adjustment in the Sample
The Temperature Controller controls the temperature of the actively heated components. The tem-
perature in the sample is affected primarily by the temperature of the Heated Plate and the Heated
Glass Bottom, and, due to heat loss to the surrounding environment, they must usually be set to a
temperature slightly above the desired temperature in the sample. The Heated Lid acts primarily
to prevent greater heat loss to the environment and to prevent condensation in the chamber during
microscopy experiments; it should generally have a temperature several degrees above that of the
Heated Plate. To ensure that the sample reaches the correct temperature, ibidi recommends a tem-
perature adjustment step before starting the first experiment. The temperature adjustment should
also be done separately for each chamber type used.
When starting the temperature adjustment in the sample in your specific setup, use the recommended
temperature settings:
Table 5 – Recommended operation parameters
Parameter
Recommended Values
Heated Lid
42°C
Heated Plate
38°C
Heated Glass Bottom
37°C
To measure and calibrate the sample temperature, use the provided Temperature Adjustment Set.
The temperature of the heated components should be adjusted in small steps. Follow this procedure
to adjust the sample temperature:
1. Connect the temperature sensor (thermocouple type K) to the plug labeled ”Temp Sensor” on
the Temperature Controller’s back.
2. Fill the provided multi-well plate with water. The volume of water and type of plate should
correspond to those of your experiment.
3. Put the loose end of the thermocouple through the hole in the lid of the multi-well plate, making
sure that it is submerged in water.
4. Place the multi-well plate onto the Heated Plate.
5. Set all external parameters, such as room temperature, air conditioning, airflow, illumination,
microscope settings, etc, to the values that you will use in your experiment. For a 37°C sample
temperature, use the recommended temperatures in table
6. After 30 minutes, check the temperature of the external sensor on the display or in the Incu-
Control software. If the sample temperature is still too low, raise the set values for the Heated
Lid, Heated Plate, and Heated Glass Bottom all uniformly by 0.5-1°C and wait until the sample
temperature is stable again (at least 10-15 min).
7. When the sample temperature has reached the desired value, write down set values for Heated
Plate, Heated Glass Bottom, and Heated Lid.
Instruction Manual
23
Version 2.0 (March 22, 2019)
