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ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
5.1
5
N
ON
-G
YNECOLOGIC
S
AMPLE
P
REPARATION
C h a p t e r F i v e
Non-Gynecologic Sample Preparation
INTRODUCTION
This chapter provides instructions for preparing non-gynecologic (non-gyn) samples and making
slides with the ThinPrep
™
5000 system.
For the best results, carefully follow the instructions in this chapter. Because there is biological
variability among samples and variability in collection methods, standard processing may not
always yield a satisfactory and uniformly distributed preparation on the first slide. This chapter
contains troubleshooting instructions for further sample processing to obtain better quality
subsequent slides in these cases. This chapter also provides an outline of various sample collection
methods and the appropriate procedures for each.
Content found in this chapter:
METHODS OF SAMPLE PREPARATION
•
Concentrate by centrifugation — 600g for 10 min.
•
Pour off supernatant and vortex to resuspend cell pellet
•
Evaluate cell pellet appearance
•
Add specimen to PreservCyt
™
Solution vial
•
Allow to stand in PreservCyt Solution for 15 min.
•
Run on ThinPrep
™
5000 processor with AutoLoader using Sequence Non-Gyn. Fix,
stain, and evaluate.
•
Mechanical agitation
•
CytoLyt
™
Solution wash
SPECIMEN PREPARATION GUIDELINES
•
Fine Needle Aspirates
•
Mucoid Specimens
•
Body Fluids
•
Urine Specimens for the Vysis
®
UroVysion assay
SAMPLE PREPARATION TROUBLESHOOTING
SECTION
A
Содержание ThinPrep 5000
Страница 1: ...Operator s Manual ThinPrep 5000 Processor with AutoLoader...
Страница 5: ...This page intentionally left blank...
Страница 6: ...Instructions For Use Instructions For Use...
Страница 43: ...Table of Contents Table of Contents...
Страница 48: ...1 Introduction 1 Introduction...
Страница 73: ...2 Installation 2 Installation...
Страница 82: ...3 PreservCyt and CytoLyt Solutions 3 PreservCyt and CytoLyt Solutions...
Страница 98: ...4 Gynecologic Sample Preparation 4 Gynecologic Sample Preparation...
Страница 107: ...5 Non Gynecologic Sample Preparation 5 Non Gynecologic Sample Preparation...
Страница 134: ...6 User Interface 6 User Interface...
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Страница 217: ...7 Operating Instructions 7 Operating Instructions...
Страница 256: ...8 Maintenance 8 Maintenance...
Страница 265: ...ThinPrep 5000 Processor with AutoLoader Operator s Manual 8 9 8 MAINTENANCE Figure 8 8 Slide Waste Bin...
Страница 282: ...MAINTENANCE 8 26 ThinPrep 5000 Processor with AutoLoader Operator s Manual 8 This page intentionally left blank...
Страница 283: ...9 Troubleshooting 9 Troubleshooting...
Страница 308: ...10 Staining and Coverslipping 10 Staining and Coverslipping...
Страница 313: ...11 ThinPrep Pap Test Training Program 11 ThinPrep Pap Test Training Program...
Страница 316: ...Service Information Service Information...
Страница 319: ...Ordering Information Ordering Information...
Страница 326: ...Index Index...
Страница 345: ...Operator s Manual ThinPrep 5000 Processor with AutoLoader...