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Hoefer TE 22, Руководство пользователя

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user

manual

TE 22

um

  

TE22-IM/Rev. E0/08-04

Hoefer

™

 TE 22

tank transfer unit

Содержание TE 22

Страница 1: ...user manual TE 22 um TE22 IM Rev E0 08 04 Hoefer TE 22 tank transfer unit...

Страница 2: ......

Страница 3: ...ophoresis Unit function and description 1 Specifications 2 Important information 4 Operating instructions 6 Care and maintenance 12 Troubleshooting 13 Electrotransfer notes 15 Bibliography and referen...

Страница 4: ...Inc 953 Indiana Street San Francisco CA 94107 USA support hoeferinc com Hoefer Inc reserves the right to make changes in the specifi cations without prior notice Warranty and liability Hoefer Inc guar...

Страница 5: ...rve le droit d effectuer des modifications de ces sp cifications sans aucun pr avis Garantie et responsabilit Hoefer Inc garantit l utilisateur que le produit livr a subi avec succ s tous les essais p...

Страница 6: ...sco CA 94107 USA support hoeferinc com Hoefer Inc beh lt sich das Recht vor die Spezifikationen ohne vorhergehende Ank ndigung zu ndern Gew hrleistung and haftung Hoefer Inc garantiert da das geliefer...

Страница 7: ...ferinc com Hoefer Inc se reserva el derecho a modificar las especifica ciones sin previo aviso Garant a y responsabilidad Hoefer Inc garantiza que el producto entregado ha sido probado a fondo para co...

Страница 8: ...3 Indiana Street San Francisco CA 94107 USA support hoeferinc com Hoefer Inc si riserva il diritto di apportare modifiche ai dati tecnici senza preavviso Garanzia e responsabilit Hoefer Inc garantisce...

Страница 9: ...er temperature can be controlled by circulating cooled liquid through the heat exchanger in the base The buffer is separated from the coolant by a heat conducting alumina plate Unpacking Unwrap all pa...

Страница 10: ...to 80 Altitude up to 2000 m Installation category II Pollution degree 2 Dimensions w h d 14 24 16 5 cm 5 5 9 5 6 5 in Product certifications EN61010 1 UL3101 1 CSA C22 2 1010 1 CE This declaration of...

Страница 11: ...electrode panels 2 electrode retaining screw 2 transfer tank up to four cassettes fit into the slots heat exchanger ports 2 heat exchanger pressure safety valve cassette hook color coded leads fill le...

Страница 12: ...ater tap or any coolant source where the water pressure is unregulated For longer runs you can control heating somewhat by chill ing the buffer before use running the unit in a cold room or both When...

Страница 13: ...lation d eau un robinet ou quelque source de refroidisse ment dont la pression n est pas r guli re Pour des coulages plus long on peut aussi contr ler la temp rature en refroidissant le tampon avant...

Страница 14: ...above atmospheric pressure Set the temperature to 10 C or higher if circulat ing only water If using 50 50 ethylene glycol water the temperature can be set lower Start the circulator bath at the same...

Страница 15: ...water Pre wet PVDF or other hydrophobic membranes in methanol Then soak all membrane types in transfer buffer for 2 5 minutes 2 Open the cassette by releasing both latch tabs along the edge opposite t...

Страница 16: ...f the stack seems tight replace the top sponge over the gel with a sheet of blotting paper If you remove the bottom sponge below the gel substitute at least two sheets of blotting paper to create spac...

Страница 17: ...the sponges Place the tray holding the cassette s near the tank lift out one cassette at a time and slide it into a set of vertical slots Do not discard the buffer 2 Once in place tap the cassette lig...

Страница 18: ...et to about 10 C If using 50 50 ethyl ene glycol water the temperature can be set lower Chill the buffer before use if possible Note Take care in orienting the lid so that all species migrate toward t...

Страница 19: ...supply Disconnect the leads from the power supply jacks 2 Lift off the lid Use the plastic hook stored in the holder at the side of the unit to lift up a cassette just far enough to be able to grab i...

Страница 20: ...llow the unit to air dry completely Periodically wash with a dilute solution of a mild detergent Removing the electrode panel s For more thorough cleaning or to replace damaged electrodes remove each...

Страница 21: ...bly instructions on page 7 Grid pattern on membrane Add extra sheets of blotting paper to increase the clearance between the cassette panel and the gel Take care not to overstuff the cassette the gel...

Страница 22: ...eters Fix or crosslink the molecule onto the membrane according to the requirements of the nucleic acid protein or membrane type Prepare protein transfer buffer without SDS Verify the optimal amount o...

Страница 23: ...s to a membrane increases the sensitivity of detection methods such as autoradiography and permits detection of specific proteins by antibodies or affinity labels and of specific nucleic acids by hybr...

Страница 24: ...s such as pH salt type and concentration and the presence of detergents such as sodium dodecyl sulfate SDS Condi tions required for efficient elution may not coincide with optimal conditions for bindi...

Страница 25: ...circulator bath set to 10 C if using water as a coolant You can use a lower setting if the coolant is 50 50 ethylene glycol water Never leave the unit unattended for more than one hour under high powe...

Страница 26: ...ol from the transfer buffer Gershoni and Palade obtained a much more quantitative transfer The disadvantage of cationic membrane is that protein stains also bind well so that the stain ing background...

Страница 27: ...ne FW 75 07 192 mM 28 8 g SDSa FW 288 4 0 1 3 5 mM 2 0 g Dissolve in 1 5 liters distilled water Add methanol as requiredb Bring to 2 liters with distilled water Do not adjust the pH which should be be...

Страница 28: ...ash gels three times 20 minutes each to assure complete removal of denaturants and detergents See Bittner et al for a study of the transfer effi ciency for DNA of different sizes The Bittner transfer...

Страница 29: ...down to 0 1X may be used should it be necessary to decrease the amount of current in the system in order to control overheating RNA transfer buffer 10X 10X Tris acetate EDTA TAE b pH 8 4 1 liter Tris...

Страница 30: ...ed nitrocellulose and radiographic detection with antibody and radioiodinated protein A Anal Biochem 112 195 1981 Gallagher S Winston S E Fuller S A and Hurrell J G R Immunoblotting and Immunodetectio...

Страница 31: ...Sci USA 76 3116 1979 Sambrook J and Russell D W Molecular Cloning A Laboratory Manual Cold Spring Harbor Labora tory Press A1 17 2001 Southern E M Detection of specific sequences among DNA fragments s...

Страница 32: ...d 1 TE24 1 foam sponge 6 mm thick Foam sponges 9 10 5 cm 6 mm thick 4 TE25 Foam sponges 9 10 5 cm 3 mm thick 4 TE25F 1 8 Electrode panel 1 TE23 Safety lid with cables 1 TE29 High voltage leads with ja...

Страница 33: ......

Страница 34: ...p27 Printed in the USA Hoefer Inc 953 Indiana Street San Francisco CA 94107 USA www hoeferinc com Contrad 70 and Decon 90 are trademarks of Decon Lab...

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