Hanna Instruments HI97769, Инструкция по эксплуатации, Страница: 22 / 32

22
METHOD PROCEDURE
• Press Start and the display will show a 2 minute countdown. To skip the timer,
press Zero . To stop the timer press Stop . During this period, the chloroform layer
separates from the aqueous layer.
• Remove the cap.
• Insert a clean plastic pipette below the upper aqueous
layer to transfer the lower chloroform layer into a
cuvette. Do not transfer any of the upper aqueous layer.
Notes: The solution in the cuvette must be clear. If the solution is cloudy, the separation
between the chloroform and aqueous layer can be improved by gently warming the
vial (holding the vial in your hand). If the chloroform layer contains some aqueous
drops hanging on the cuvette wall, gently swirl or invert the cuvette. It is important to
transfer at least 7 mL of chloroform layer into the measurement cuvette, thus up to
0.5 cm (1/4”) below the 10 mL mark. If the transferred volume is lower than 7 mL,
the accuracy of the test may be affected. Please repeat the test waiting for longer than
2 minutes to allow complete separation between the two phases.
• Replace the plastic stopper and the cap. This is the
reacted sample (#2).
• Fill another cuvette with 10 mL of Chloroform
Reagent (up to the mark). Replace the plastic
stopper and the cap. This is the blank (#1).
• Insert the blank (cuvette #1) into the holder and
ensure that the notch on the cap is positioned
securely in the groove.
#2 sample
#1 blank
#1