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Germfree - Class II, Type A2 Biosafety Cabinet User Manual - v2.1 - 09SEP2021 - RMH

Class II, Type A2 Biosafety Cabinet

User Manual

Содержание BBF 2

Страница 1: ...Germfree Class II Type A2 Biosafety Cabinet User Manual v2 1 09SEP2021 RMH Class II Type A2 Biosafety Cabinet User Manual ...

Страница 2: ... Cleaning the Viewing Panel 9 Stainless Steel Work Deck 10 Motor Blower 11 Lights 12 Performance Testing 13 Use of the Biosafety Cabinet 14 Advance Planning 14 Good Technique 14 Biological Safety Cabinet Operation Don ts 15 Troubleshooting 16 Dimensions 18 Parts List 19 Quality Statement 23 Our Company 23 Germfree s Mission 23 Appendix A Line Drawings Appendix B Wiring Diagrams Appendix C Sensocon...

Страница 3: ...th 99 99 efficiency and they even more efficient for both larger and smaller particulates Perhaps the most valuable parameter of the Class II Type A2 Biosafety Cabinet is containment without the necessity of working through gloves This is accomplished by discharging approximately 30 of the total filtered air each cycle through the exhaust HEPA filter in the top of the unit An equal amount of make ...

Страница 4: ... fit through any opening 32 wide and 58 high If narrower doors are encountered the light housing can be taken off by removing four screws Alternatively the viewing panel with the attached light housing can be completely removed by sliding the split hinges to the right Removing this panel will reduce the depth of the biosafety cabinet to 28 2 units will fit through any standard doorway ...

Страница 5: ...ropriate 3 prong 115v receptacle For convenience of operations and service all circuitry is located within the control panel Standard services include the Motor ON OFF switch light switch a Sensocon or Magnehelic see front cover image gauge at the front of the unit and a speed control located in the control panel behind a plastic cap Optional duplex electric outlets may be located in the rear of t...

Страница 6: ...w fpm Volume cfm Exhaust Flow fpm BBF 2 87 102 65 80 114 134 154 181 BBF 3 102 112 67 82 147 161 123 134 BBF 4 102 112 67 82 215 236 130 142 BBF 6 102 112 70 85 353 388 106 117 Eventually the HEPA filters will be loaded with particulates to a point where the blower can no longer overcome the resistance to provide the proper air velocities At this point new HEPA filters must be installed Replacemen...

Страница 7: ...Class II Type A2 Biosafety Cabinet User Manual 7 Airflow Diagram ...

Страница 8: ...vice department can assist with or perform most repairs Certification and Repair All models of Class II Type A2 Biosafety Cabinets have a large supply and a smaller exhaust HEPA filter These are sized to provide the entire work area with ultra clean air and to filter particulate matter Approximately 30 of the circulated air is exhausted during each cycle HEPA filters are placed and sealed within t...

Страница 9: ...plug the light housing cord from the electrical outlet located at top right corner and remove the small snap ring from the right most hinge If the unit has shocks they must be detached prior to removal Next raise the viewing panel until it is parallel to the floor and slide the panel toward the right side completely separating the split hinges Be sure to replace snap ring after use Cleaning the Vi...

Страница 10: ...rated support and the rear and side walls can be decontaminated by using a sponge or squeeze bottle containing a biocide specific to the organisms being worked with Spilled material within the chamber should be made safe by the addition of an appropriate germicide followed by the appropriate cleaning procedures For initial cleaning and daily maintenance the interior of the chamber should be comple...

Страница 11: ...aterial Motors are 1 3 or 1 2 h p 115 V single phase and 60 Hertz Before servicing the motor the power cord must be disconnected In the Class II Type A2 Biosafety Cabinet access to the motor blower is through the front of the unit If potentially hazardous material has been handled the biosafety cabinet should first be decontaminated If the unit is used to contain non biological chemical hazards th...

Страница 12: ... own housing along the top of the viewing panel and exterior to the work area This location gives the best glare free view to the operator and also prevents heat build up within the cabinet The lamps are wired independent of the motor blower and provide good visibility within the work area at all times ...

Страница 13: ...t PAO or equivalent aerosol particles on the downstream side of the HEPA filters If there was any penetration greater than 01 through the filter medium or perimeter filter seal the leaks were located and repaired Air velocity was measured with a thermoanemometer to insure that it was uniform and unidirectional Other tests for noise level vibration level and light intensity were performed the Class...

Страница 14: ...work area so they do not block the air intake or the return air openings Perform work far enough away from the access opening i e over the work tray to insure good containment Wait approximately ten minutes after starting the unit before initiating procedures This allows sufficient time for the ultra clean air circulating within the chamber to remove any airborne contamination from the work area a...

Страница 15: ...lassware etc can be wiped or sprayed with the appropriate solution Wait approximately five minutes before removing any item from the chamber Biological Safety Cabinet Operation Don ts 1 DON T ever cover the air intake area in front of the chamber with papers notebooks equipment etc Movement of air into the negative pressure section must not be impeded 2 DON T ever cover the exhaust filter 3 DON T ...

Страница 16: ...tch should be on No air circulation Blower off Check motor blower control Blown circuit breaker Check circuit breaker on control panel Break in electrical wiring Use electrical diagram and check wiring connections Excessive blower vibration Foreign matter in blower squirrel cage Remove paper paper clip etc Exhaust air imbalance Obstruction over exhaust filter Remove obstruction Improper adjustment...

Страница 17: ...ion Leak between blower and plenum Repair leak contact certification company High velocity air Exhaust filter rupture Replace Filters Contact certification company Supply filter rupture Repair contact certification company Supply filter gasket leak Find and repair leak contact certification company Exhaust air outlet obstruction Remove obstruction Improper adjustment of blower motor speed control ...

Страница 18: ... 46 BBF 2SSRX 24 23 54 BBF 3SSRX 36 27 57 BBF 4SSRX 48 27 57 BBF 6SSRX 72 27 57 Light housing and control panel adds an additional 4 to depth Depth dimensions include light housing Work Area Dimensions Width Depth Height BBF 2SSCH 23 17 21 BBF 2SSRX 23 17 30 BBF 3SSRX 35 22 28 BBF 4SSRX 47 22 29 BBF 6SSRX 71 22 29 ...

Страница 19: ...BBF 2SSRX 20 x 20 x 3 375 1001 00 18 x 8 x 6 375 1002 00 BBF 3SSRX 20 x 34 x 6 375 1004 00 18 x 12 x 6 375 1003 00 BBF 4SSRX 20 x 46 x 6 375 1006 00 18 x 16 x 6 375 1005 00 BBF 6SSRX 20 x 70 x 6 375 1007 00 2 18 x 16 x 6 375 1005 00 Blowers All models Direct Drive forward curved LAU Industries DD97 AT 003 1400 00 Viewing Panel BBF 2SSCH BBF 2VPC Acrylic plastic 625 0006 00 BBF 2SSRX BBF 2VP Acryli...

Страница 20: ...RX BBF 3SSRX BBF 4SSRX 1 3 hp 1625 rpm 5 7 amps Fasco 7124 1126 1 2 182 0010 00 BBF 6SSRX 183 0043 00 Diffusors BBF 2SSCH BBF 2SSRX 009 1100 02 BBF 3SSRX 009 1100 03 BBF 4SSRX 009 1100 04 BBF 6SSRX 009 1100 06 IV Bars Bar All Models 003 6001 00 Bracket All Models 003 1000 10 ...

Страница 21: ... Contact Customer Service Switches All models 10A 250 vac 12A 125 vac UL Approved CARLING SWITCH PART T1GA21 6S BL NBL 170 0030 00 Motor Speed Control BBF 2SSCH BBF 2SSRX BBF 3SSRX BBF 4SSRX 15 AMP 115V UL Approved KB ELECTRONICS PART KBMC 115NS 332 0014 00 BBF 6SSRX 332 0043 00 Circuit Breaker All Models UL CSA Approved POTTER BROOMFIELD PART W58 XB1A4A XX XX RATED AMPS ...

Страница 22: ...BBF 4SSRX L BBF 4SSRX BBF 6SSRX L BBF 6SSRX Fluorescent Ballast All models Advance Transformer Ballast 1CN 2P32 SC35M 147 5007 00 Fluorescent Tubes BBF 2SSCH BBF 2SSRX F 17T8 841 BBF 3SSRX F 25T8 741 BBF 4SSRX 32T8 741 BBF 6SSRX 32T8 741 Fluorescent Lamp Holders All Models 660W 600V UL Approved LEVINGTON PART 13057 UN ...

Страница 23: ... as permanent facilities in remote regions with harsh conditions Quality Statement Accountability We will deliver our products on time as promised and free from defects Ownership We will strive to exceed expectations at every level and we will work to make sure that each customer is satisfied with the service that they receive Longevity All of our products are constructed from the highest quality ...

Страница 24: ...Class II Type A2 Biosafety Cabinet User Manual Appendix A Line Drawings ...

Страница 25: ... Drawings BBF 2 E GERM 003 2000 00 dft Germfree Laboratories UNLESS OTHERWISE SPECIFIED TOLERANCES ARE XX 03 XXX 010 FRACTIONS 1 16 ANGLES 5 DIMENSIONS ARE IN INCHES DO NOT SCALE DRAWING SHEET NAME DRAWN BY APPROVAL XX XX XX 05 08 20 B SERLE T JAFA CHECKER B SERLE XX XX XX N A MATERIAL FINISH N A N A REVISIONS Rev Description Date Drawn By Checked By Approved A INITIAL RELEASE xx xx xx XXX XXX XXX...

Страница 26: ... BBF Manual Drawings BBF 2CH E GERM 003 2200 00 dft Germfree Laboratories UNLESS OTHERWISE SPECIFIED TOLERANCES ARE XX 03 XXX 010 FRACTIONS 1 16 ANGLES 5 DIMENSIONS ARE IN INCHES DO NOT SCALE DRAWING SHEET NAME DRAWN BY APPROVAL XX XX XX 05 11 20 B SERLE T JAFA CHECKER B SERLE XX XX XX N A MATERIAL FINISH N A N A REVISIONS Rev Description Date Drawn By Checked By Approved A INITIAL RELEASE xx xx x...

Страница 27: ...anual Drawings BBF 3 E GERM 003 3000 00 dft Germfree Laboratories UNLESS OTHERWISE SPECIFIED TOLERANCES ARE XX 03 XXX 010 FRACTIONS 1 16 ANGLES 5 DIMENSIONS ARE IN INCHES DO NOT SCALE DRAWING SHEET NAME DRAWN BY APPROVAL XX XX XX 05 08 20 B SERLE T JAFA CHECKER B SERLE XX XX XX N A MATERIAL FINISH N A N A REVISIONS Rev Description Date Drawn By Checked By Approved A INITIAL RELEASE xx xx xx XXX XX...

Страница 28: ...anual Drawings BBF 4 E GERM 003 1000 00 dft Germfree Laboratories UNLESS OTHERWISE SPECIFIED TOLERANCES ARE XX 03 XXX 010 FRACTIONS 1 16 ANGLES 5 DIMENSIONS ARE IN INCHES DO NOT SCALE DRAWING SHEET NAME DRAWN BY APPROVAL XX XX XX 05 08 20 B SERLE T JAFA CHECKER B SERLE XX XX XX N A MATERIAL FINISH N A N A REVISIONS Rev Description Date Drawn By Checked By Approved A INITIAL RELEASE xx xx xx XXX XX...

Страница 29: ...anual Drawings BBF 6 E GERM 003 6000 00 dft Germfree Laboratories UNLESS OTHERWISE SPECIFIED TOLERANCES ARE XX 03 XXX 010 FRACTIONS 1 16 ANGLES 5 DIMENSIONS ARE IN INCHES DO NOT SCALE DRAWING SHEET NAME DRAWN BY APPROVAL XX XX XX 05 08 20 B SERLE T JAFA CHECKER B SERLE XX XX XX N A MATERIAL FINISH N A N A REVISIONS Rev Description Date Drawn By Checked By Approved A INITIAL RELEASE xx xx xx XXX XX...

Страница 30: ...Class II Type A2 Biosafety Cabinet User Manual Appendix B Wiring Diagrams ...

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Страница 32: ......

Страница 33: ...Class II Type A2 Biosafety Cabinet User Manual Appendix C Sensocon User Manual ...

Страница 34: ...Bulletin 102 0 Installation and Operation Manual Series A3 and A4 Differential Pressure Controller Sensocon Inc Phone 863 248 2800 Fax 863 248 2798 www sensocon com ...

Страница 35: ... Specifications 1 3 Dimensional Drawings 2 Installation 2 1 Mounting 2 2 Pressure Connections 2 3 Electrical Connections 3 Operation 3 1 Display 3 2 Key Function 4 Programming 4 1 Menu Structure 4 2 Security Level 4 3 Main Menu 4 4 Set Up Menu 4 5 Secure Menu 5 Other Features 5 1 Moving Program Variables 5 2 Opti Link 5 3 Limited Warranty ...

Страница 36: ...utput and improved accuracies There is also a low cost weatherproof cover available for outdoor applications Example A3110 06 Description Model A Round Panel Mount Housing 3 LED display process arch LCD Display 4 LED display process arch OLED Control 1 Control 2 SPDT relays 0 None Transmitter 1 4 20 mA 0 1 Accuracy 1 0 50 01 0 0 50 02 0 1 03 0 2 04 0 3 05 0 4 06 0 5 07 0 8 08 0 10 09 0 15 10 0 20 ...

Страница 37: ...ce with optional cover the entire product is weatherproof Relays 2 SPDT 8 Amps 250 VAC 5A 30 VDC Electrical Connections screw terminals Response Time 100 ms Display 4 Digit red LED digits LCD or OLED programming display Process Connection Push on connection for 3 16 tubing Agency Approvals Pending UL C UL CE Patents Pending Custom calibrations including improved accuracy and improved thermal effec...

Страница 38: ...control with the provided gasket through the hole and secure it to the panel with the provided mounting tabs and screws Retrofitting old technology is also easy with the Series A3 and A4 They have been designed to fit in industry standard holes ranging from 4 9 16 to 4 13 16 so simply remove the old device and insert the new control into the existing cut out 2 INSTALLATION 2 1 Mounting ...

Страница 39: ...he weather proof cover has been attached the control can be mounted to any flat surface with the four mounting screws provided with the cover Flush Mounting with Weather Proof cover The Series A3 and A4 can also be flush mounted with a weather proof cover The procedure is the same as above but utilizes two extra long mounting screws provided with the weather proof cover for the bottom two panel co...

Страница 40: ...the front display will give a positive value If the HI connection has a lower absolute value than the LO connection the front display will give a negative value NOTES 1 Supply Voltage is universal from 16 265VAC or VDC 2 For internally sourced 4 20 mA loop maximum loop resistance is 750 Ω for externally sourced loop at 36 VDC maximum loop resistance is 1800 Ω 3 Isolation Relays 1000 VAC to all oth...

Страница 41: ...show either 3 or 4 digits based on the users preference The LCD or OLED display is used for programming and displaying the unit of measure and up to 4 other user selected program parameters The LED indicators for SP1 SP2 and Alarm give indication when the parameter is in the actuated state The A3 and A4 have four buttons located on the face of the control used for programming There is an up arrow ...

Страница 42: ...he accept button for three seconds this will accept the change and take you back to the menu you were currently in At any point while in a parameter change screen you can hold the cancel button for one second to return to the previous menu without accepting a change to that parameter To manually reset the alarm after an alarm condition has occurred if this feature is used hold the right and left a...

Страница 43: ...p value Screen Saver Saver Off On Contrast Contr N lo N med N hi I lo I med I hi K Factor Kfact value Duct Shape Xsect circ rect Duct Diameter diam value Duct Width X dim value Duct Height Y dim value Zero Zero Reset Span Span value Reset Access Access Main Set up Secure none Factory Default Reset No Yes Process Output Lo pol value Process Output Hi poh value Secure Menu Current Power source P S O...

Страница 44: ... Set Point is selected there will be no parameters for Set Point 2 or Alarm in the programming 1 Set Point 1SP For control with one SPDT relay 2 Set Point 2SP For control with two independent SPDT relays Set Point Alarm SPAL For control with one SPDT relay and one alarm relay Alarm AL For alarm operation only Set Point Settings Set 1 Set 2 Each set point can be entered as a high and low value for ...

Страница 45: ...OFF OFF ON ON ON Relay Action Direct Acting Reverse Acting DB direct acting DB reverse acting Actuation 1 act 2 act The actuation parameter determines whether the relays will react to increasing or decreasing pressure This parameter also affects the status of the LED indicators on the front of the gauge The above graphs illustrate the differences Direct The relays turn on with increasing pressure ...

Страница 46: ...e the relay when the process is below the low alarm set point The high and low alarm can be used together so the alarm relay is actuated when the process is above or below the high alarm and low alarm set points respectively The selection will dictate which alarm parameters are shown in the Main Menu The below figure shows the three alarm types High Alarm hi High alarm Low Alarm lo Low alarm High ...

Страница 47: ...ed since the last time it was reset value Allows the user to manually reset the value Mode Mode The control is capable of measuring and displaying pressure velocity or flow For velocity and flow a flow sensing device such as a pitot tube or orifice plate with a know flow coefficient is required For flow it is also necessary to know the dimensions of the duct that the flow is being measured in so t...

Страница 48: ...40 0 036 0 074 1 868 2 491 249 1 0 249 0 578 2 000 0 167 50 80 5 080 0 072 0 147 3 737 4 982 498 2 0 498 1 156 3 000 0 250 76 20 7 620 0 108 0 221 5 605 7 473 747 3 0 747 1 734 4 000 0 333 101 6 10 16 0 145 0 294 7 473 9 964 996 4 0 996 2 312 5 000 0 417 127 0 12 70 0 181 0 368 9 342 12 45 1245 1 245 2 890 8 000 0 667 203 2 20 32 0 289 0 588 14 95 19 93 1993 1 993 4 624 10 00 0 833 254 0 25 40 0 3...

Страница 49: ...ntrast Contr This allows the image and contrast of the programming display to be adjusted Normal low N lo Normal image low contrast Normal medium N med Normal image medium contrast Normal high N hi Normal image high contrast Inverted low I lo Inverted image low contrast Inverted medium I med Inverted image medium contrast Inverted high I hi Inverted image high contrast K Factor Kfact For flow and ...

Страница 50: ...revent tampering Once this parameter is set a security code must be entered to regain access to the locked menus as shown in the below table This feature can be used in conjunction with moving program variables section 5 1 to grant or restrict access to specific variables Secure menu Secure Selecting Secure will give the user access to all menus Set up menu Set up Selecting Set Up will give the us...

Страница 51: ...current loop The programming menu can be fully customized by the user Parameters can be moved from one menu to another This allows the user to reorganize the menu structure to better fit their needs or to put unused or unwanted parameters in one menu and then lock that menu so that those variables can not be accessed To move a program parameter to another menu highlight the parameter to be moved a...

Страница 52: ...te the program has been successfully uploaded to the control LIMITED WARRANTY SENSOCON warrants its products to be free from defects in materials and workmanship for a period of one 1 year from the date of shipment subject to the following terms and conditions Without charge SENSOCON will repair replace or refund the purchase price at SENSOCON s option products found to be defective in materials o...

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