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Genevac HT-24

User Manual

50

10-1562 Issue 3-7 March

2014

Condensation

Solvent condensation within the evaporator is most likely to occur with high boiling point
solvents such as NMP, DMI, DMSO and possibly DMF. It occurs when the chamber walls
are cooler than the solvent vapour.

To prevent this happening, the evaporator chamber should be pre-heated. To do this, enter
the

Run Data

screen, set the

Minimum Chamber Temperature

field to 40°C and start the

run.  From room temperature, the chamber may take up to 40 minutes to reach this
temperature.  Note the CoolHeat lamps do not operate during this pre-heating cycle.

Do not pre-heat the chamber when evaporating volatile solvents such as TFA, acetonitrile or
methanol.Check the sample swings are able to move freely.

Clean the quartz glass using a suitable solvent (such as methanol or acetone) and a lint free
cloth or paper towel. Clean the swings and rotor in the same way.  Take care to avoid
solvent contact with the outside paintwork and accessories of the chamber.

TFA creep

TFA

creep

is the movement of TFA up the inside of the tubes, vials or microtitre plates.

Cross contamination problems can arise and sample material may be thrown onto the IR
lamp windows as the rotor spins. Deposits on the lamp lenses can be carbonised by the
heat from the lamps, causing crack propagation sites to form on the quartz glass. Inspect
the lamp glass at regular intervals and clean with a lint free cloth and acetone.

Carbonised deposit from samples

IR lamp glass cracked

A range of specialised sample holders is available to protect the quartz lenses. Contact
Genevac Sales for details.

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Содержание HT-24

Страница 1: ...Genevac HT 24 Evaporation System User Manual Original instructions Part number 10 1562 Issue 3 7 March 2014...

Страница 2: ......

Страница 3: ...ent container 32 Loading Samples 34 Sample holders 34 Use of correct accessories 34 Mass limit 35 Sample holders and swings 35 Loading the rotor 36 Balancing the rotor 37 Method guide 38 Pre programme...

Страница 4: ...with the option of an integrated fume hood The workstation also accommodates two condensers a high vacuum scroll pump and a waste solvent container Scroll pump catch pot door open Workstation front vi...

Страница 5: ...ing each evaporator independently to a PC Rear view with fume hood rear panel removed for clarity Two data connections waste solvent pump Three mains power supply connections electrical circuit breake...

Страница 6: ...load samples select the appropriate run and press start Evaporator screen and keypad Evaporation progress is shown by a graphical display with plots for temperature and pressure The evaporator control...

Страница 7: ...evaporation system Only operate the system in a ventilated space Make sure exhaust ventilation hoses are securely fitted and take solvent vapours away from the equipment and from the operator Only us...

Страница 8: ...tion Only operate the system in a well ventilated environment and consider safety when evaporating any combustible solvents Genevac s position regarding the evaporation of such solvents particularly w...

Страница 9: ...ar fumes before opening the fume hood doors In addition the following optional specifications can be added at the time of ordering Consult your Genevac representative for advice on any other requireme...

Страница 10: ...shows just a few of the sample holders available Sample holders are manufactured in solid aluminium and are anodised to improve heat absorption The contact area is maximised to provide good physical s...

Страница 11: ...o power the system down on a regular basis Note The workstation power switch can be left on at all times Power distribution to the entire system can be controlled the evaporator power switches To powe...

Страница 12: ...perature The system will not allow an evaporation run to commence until the condenser reaches 0 C if an attempt is made to start the run will commence once operational temperature has been reached To...

Страница 13: ...e evaporator doors before closing the fume hood doors Warning Risk of hand entrapment between the left hand evaporator and the side of the fume cupboard Take care when opening the evaporator door Stan...

Страница 14: ...to open evaporator 2 right hand side evaporator 3 ON indicator Lights up to indicate workstation is powered 4 EMERGENCY STOP Press to isolate the workstation and stop both evaporators in an emergency...

Страница 15: ...show different segments in time 5 SETTINGS Press to display the Run Data screen for the selected run 6 Keypad Enter data for setting up or editing runs 7 Evaporator power switch Controls distribution...

Страница 16: ...y selected method number 2 Status shows current evaporator status 3 Graphical data Shows progression of evaporation run with data for Channel 1 temperature Channel 2 temperature Pressure and Spin Rate...

Страница 17: ...SampleGuard channel 2 is optional SampleGuard probes are available in lengths to suit different types of sample holder Caution Risk to sample integrity make sure the probes are long enough to allow t...

Страница 18: ...contact your local Genevac representative to request a Genevac Accessories Brochure or visit the Genevac website at www genevac com Selecting a run From the SELECT RUN screen use the cursor direction...

Страница 19: ...races begin to converge this indicates the sample is approaching dryness Run parameters can be changed whilst the evaporator is operating refer to Advanced Operation Setting up a run Stopping a run To...

Страница 20: ...ed Skip The evaporator returns to the Run Select screen Drain The system opens the drain valve and pumps any waste solvent into the waste solvent container Defrost Enter the defrost menu Select START...

Страница 21: ...d The following section describes how to set up a new method the process is similar for overwriting an existing method Begin by selecting an empty slot from the SELECT RUN screen or select an existing...

Страница 22: ...xt Run Number Runs can be linked together enter the number of the run that requires to be linked and it will commence when the current run finishes Any number of runs can be linked together in this wa...

Страница 23: ...selected solvents are highlighted in blue Select Done when the solvent or solvents have been selected For solvents that are not listed select Other and enter the solvent name manually no more than 31...

Страница 24: ...emaining run parameters Auto programmed run parameters are shown in blue but can be manually overwritten in which case they revert to being shown in white Alternatively select N No to continue enterin...

Страница 25: ...SampleGuard Control Channel Channel 1 is the default setting The option to change to channel 2 is provided only to allow continued operation should a fault arise with channel 1 or its associated Samp...

Страница 26: ...cuum chamber to cool before evaporating volatile solvents or mixtures that may be prone to bumping this is unnecessary for most applications function Automatic Control To optimise the cryo pumping eff...

Страница 27: ...ly if Dri Pure is selected see Pressure Control Regime Low Samples are subject to lower centrifugal force approximately 350 G This should be used for applications that do not require high rotor speed...

Страница 28: ...uired to maintain the sample holders at control temperature When sample dryness is detected the system automatically turns off the IR lamps and performs a final drying stage before stopping the run Sa...

Страница 29: ...The evaporator selects high rotor speed and reduces the chamber pressure slowly The IR lamps do not switch on during the Dri Pure ramp Select this setting if bumping is anticipated Variable Dri Pure...

Страница 30: ...ges This Stage time elapsed since start of current stage changes to End of Run or End of Stage and begins to count down when final run time is known Graphical display for Channel 1 Temperature Channel...

Страница 31: ...nu screens Menu bar The default position for the horizontal menu bar cursor is with START highlighted Press the cursor direction left and right keys to return the cursor to START after using any of th...

Страница 32: ...ta key evaporator methods will remain unchanged all other methods will be overwritten COPY ALL METHODS TO DATAKEY Methods can be copied for safekeeping or for cloning another system COPY LOG FILES TO...

Страница 33: ...in one operation but using automatic defrost and drain methods can be set up to perform multiple operations without the need for manual intervention between stages Automatic defrost and drain also hel...

Страница 34: ...e will continue until the system senses that all solvent in the condenser pot is liquid or can be stopped manually by pressing the STOP button To drain liquid solvents from the condenser without going...

Страница 35: ...urn counter clockwise to release the drain pipe then pull the drain pipe out of the container Pull the breather pipe out of the container and disconnect the electrical earth strap from the container l...

Страница 36: ...le contact your local Genevac representative and request a Genevac Accessories Brochure Use of correct accessories Take care to make sure that the correct sample holders are used follow this guidance...

Страница 37: ...per swing This is the total mass that can be loaded onto each position of a swung rotor and includes The swing The sample holder The tubes or vials The sample solutions Do not exceed the mass limit un...

Страница 38: ...tribute tubes symmetrically in sample holders Make sure tubes or vials are inserted fully into the sample holder wells If using separate swings make sure the sample holders are located correctly in th...

Страница 39: ...tribute the samples into four holders to help distribute the heat flow evenly between samples and to reduce mechanical stress on the rotor Alternatively two empty swings can be placed on the unused ro...

Страница 40: ...cetate or methanol Single volatile solvents with no risk of bumping 52 Very volatile DCM 40 to 90 C DCM methylene chloride or chloroform Volatile solvent mixtures and DCM Methylene chloride where risk...

Страница 41: ...lyophilisation methods it will also indicate when the monitored sample has frozen and when the lyophilisation process ends Use the data to optimise the method for example if it can be proven that a f...

Страница 42: ...yl chloride SOCl2 Dichloromethane DCM Toluene Diisopropyl ether DIPE Trichloroacetic acid TCA Dimethyl acetamide Triethylamine Et3N N N Dimethyl formamide DMF Trifluroacetic acid TFA Dimethyl sulfoxid...

Страница 43: ...orate a mixture of DMSO and DCM Specific method settings would depend on variables such as volume mixture ratio and the type sample holder used 1 Select Variable Dri Pure and set a ramp from 750 mbar...

Страница 44: ...Your Volume Your Volume Your Volume Solvent MeOH H20 MeOH H20 MeOH H20 SampleGuard Control Temp C 40 40 40 SampleGuard Control Channel 1 1 1 CoolHeat Enable Pressure mbar 50 50 50 Chamber Temperature...

Страница 45: ...40 0 40 SampleGuard Control Channel 1 1 1 CoolHeat Enable Pressure mbar 50 50 50 Chamber Temperature control c preheat to 20 preheat to 0 preheat to 30 Rotor Speed High High Low Heat Off Option Elapse...

Страница 46: ...r Volume Your Volume Solvent DMSO or DMF DCM DMSO or DMF DCM DMSO or DMF DCM SampleGuard Control Temp C 40 40 40 SampleGuard Control Channel 1 1 1 CoolHeat Enable Pressure mbar 50 50 50 Chamber Temper...

Страница 47: ...e of solvent is evident on the inside of the evaporation chamber or if any solvent is spilled For further instructions refer to the following sections General cleaning and Sample holders Periodic main...

Страница 48: ...as been in contact with biological agents a bleach solution may be used IR lamp lenses Using acetone applied to a paper towel or cloth remove all traces of solvent deposit from the IR lamp lenses Depe...

Страница 49: ...ll not affect performance however if there is any structural damage if any part of the swing or sample holder is bent or deformed or if there is any sign of a crack do not use it Contact Genevac Servi...

Страница 50: ...l position The back of the system may be positioned against a wall although provision must be made for maintenance access Leave sufficient space on either side of the workstation to allow the waste so...

Страница 51: ...the unlikely event that an error occurs that that could affect the physical condition of the evaporator or its ability to safeguard the integrity of the samples a critical error the system will stop...

Страница 52: ...ol Check the sample swings are able to move freely Clean the quartz glass using a suitable solvent such as methanol or acetone and a lint free cloth or paper towel Clean the swings and rotor in the sa...

Страница 53: ...power In the event of power failure the vacuum system vents automatically the rotor may take up to two minutes to stop spinning Once the rotor is stationary and the vacuum system is at atmospheric pr...

Страница 54: ...fault this is normal Wait a few minutes for the condenser to reach operating temperature Condenser not ready after 10 minutes Contact Genevac service Out of balance error Rotor imbalance detected at...

Страница 55: ...er fails to drain Possible failure of condenser drain pump or blockage of drain pump filter Contact Genevac Service for assistance Samples not dry on lower rotor levels only Lamp layers disabled Go to...

Страница 56: ...e Sea level to 12000 m Operating environment Ambient temperature 15 C to 30 C Relative humidity 10 to 60 non condensing Altitude Sea level to 1600 m Ingress protection rating IP30 Note This evaporator...

Страница 57: ...0 Safety requirements for electrical equipment for measurement control and laboratory use General requirements BS EN 60204 1 2006 A1 2009 Safety of machinery Electrical equipment of machines General r...

Страница 58: ...ailed in the Maintenance section of this User manual Power leads supplies with the system are for use with that system only If items are replaced by the owner only Genevac approved parts may be used I...

Страница 59: ...release only update waste solvent container instructions for manual release connector add Troubleshooting note 01 May 12 3 4 Power specifications revised Screen shots updated to reflect software chan...

Страница 60: ...oing to press and may be subject to change without notice Some of the features and software functions described within this user manual may not apply to equipment manufactured before this manual s pub...

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