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9.13 Delay volumes
Introduction
A number of methods, both theoretical and experimental, exist for determining the delay volume of a
system. The easiest and recommended method is to perform a theoretical determination. Delay volumes
for standard configurations are listed in Standard delay volumes, on page 496.
Explanation of delay volume
The delay volume is the volume between the detector and the fraction collector or outlet that is used.
The illustration below shows and example of the delay volume between the UV/Vis monitor and the
fraction collector.
UV/Vis
pH
Valve
The time when a peak is detected
by the UV/Vis detector is called T
0
The peak reaches the fraction
collector at time T
1
Fraction collector
Conductivity
Delay volume
Theoretical determination of delay volumes
A theoretical determination is performed as described in the steps below:
Action Step
Identify all components in the system flow path that contribute to the delay volume of
interest. 1
Determine the internal volumes of all hardware modules and tubing, see Section 9.14
Component volumes, on page 499 for information about theoretical module volumes and
Section 9.3 Tubing and connectors, on page 420 for information about tubing lengths and
dimensions. 2
To obtain the total delay volume, sum up half of the flow cell volume of the monitor used
(that is, the UV or UV/Vis monitor) with all volumes of tubing and modules that are located
after the monitor in the flow path.
Note:
For pH-valve V9-pH and V9H-pH always use the volume for the valve in bypass position.
The system automatically adds the volumes for the flow restrictor and the pH flow cell
when if they are part of the system.
3
494 ÄKTA pure User Manual 29119969 AB
9 Reference information
9.13 Delay volumes