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9.9.3 Manual instructions - Monitors
The table below describes the monitor related manual instructions available for ÄKTA pure.
Description Instruction name
Auto zero UV sets the UV signals from U9-M or U9-L to 0 mAU. Auto zero UV
Auto zero UV 2nd sets the UV signal from UL-9, 2nd to 0 mAU. Auto zero UV 2nd
Wavelength sets the wavelengths for UV monitor U9-M. The cor-
responding curves in the chromatogram are marked with the set
wavelengths.
Note:
• The instruction is available when UV monitor U9-M (variable) is
selected in the component list. For best performance, do not
use more wavelengths than necessary for the application.
• At low wavelengths, the eluent can have absorbance of its own.
Wavelength
Noise reduction UV filters the noise in the UV signal from UV
monitor U9-M or U9-L. A column-specific averaging time is set
automatically when a column is defined in a method run and Av-
eraging time is set as a variable. Noise reduction UV
Noise reduction UV 2nd filters the noise in the UV monitor signal
from U9-L, 2nd. A column-specific averaging time is set automat-
ically when a column is defined in a method run and Averaging
time is set as a variable. Noise reduction UV 2nd
Relative scale cond facilitates monitoring of a gradient, for which
the user sets the conductivity values for 0% and 100%. The Rela-
tive scale cond can be set in ascending manner (0% for low and
100% for high conductivity) or in descending manner (0% for high
and 100% for low conductivity).
Note:
The Relative scale cond in descending manner is especially useful
for conductivity visualization in RPC and HIC, where the conductiv-
ity curve is reversed compared to the concentration curve (i.e., high
conductivity at 0% B and low conductivity at 100% B).
Relative scale cond
ÄKTA pure User Manual 29119969 AB 473
9 Reference information
9.9 Manual instructions
9.9.3 Manual instructions - Monitors