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9.7.3 Predefined phases
The table below describes the predefined phases.
Description Phase Name
The first, and mandatory, phase in any method. Defines common parameters
used in the subsequent phases. Method Settings
The Method Settings phase defines:
1 Column type
Note:
The Column type list can be filtered in two steps:
• Select the chromatography technique to be used in the list Show by
technique
• Select Show only suggested columns to show the columns that are
suggested for the selected chromatography technique.
2 Pressure limit
3 Flow rate
4 Option to control the flow to avoid overpressure
Note:
Default values for pressure limits and flow rate are given for the selected column
type.
• Column position
• Flow restrictor use
• Unit selection for Method base and Flow rate
• Monitor settings:
- pH monitor
- Air sensor alarm settings
- UV monitor
Note:
The first wavelength of U9-M and the fixed wavelength for U9-L or U9-
L 2nd is always turned on. The second and third wavelengths for UV
monitor U9-M can be set on or off.
• Settings for Column Logbook
• Start Protocol
• Result name and location
Note:
Some of these options may not be required by certain methods.
ÄKTA pure User Manual 29119969 AB 447
9 Reference information
9.7 Predefined methods and phases
9.7.3 Predefined phases