10.2.6
Block 5: Equilibration
The next block is for equilibrating the column:
Action
Step
Create a new block named
MyEquilibration
, and set its base to Same
as main (which in this case is column volumes).
1
Note:
Selecting Same as main instead of Column volumes, ensures that the volume
used is the same as that of the column you selected initially.
Set a pressure alarm at 2 bar by in-
serting the instruction
Alarm_Delta_PT111_PT112 at 0 col-
umn volumes. Make sure the alarm
is Enabled.
2
Add a Column instruction and set the column Inline.
3
Set a Flow instruction to 300 cm/h,
by first selecting Linear flow to
change unit and then typing 300. In-
sert the instruction at 0.0 column
volumes.
4
Set End_Block to 3.00, which means that the equilibration will finish after
3 column volumes. To achieve reasonable equilibration at least 1.5 column
volumes should be pumped through the column.
5
In the text pane, the finished MyEquilibration
block should look like that in the figure to the
right.
160
ÄKTA ready Operating Instructions 28960345 AD
10 Creating a method
10.2 An example of using the Method Editor
10.2.6 Block 5: Equilibration
