GE AKTA avant, Руководство пользователя

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Included phases Principle Predefined maintenance
method
The column is filled with a cleaning solu-
tion. Select inlet positions. Enter the solu-
tion identity, volume, flow rate and incuba-
tion time. By adding steps, several cleaning
solutions can be used. Suggestions for
cleaning steps are available for a number
of column types. Column CIP
After equilibration of the column, sample
is injected via a loop and eluted isocratical-
ly. A non-adsorbing sample like acetone
or salt should be used. After the run, calcu-
late column performance in the Evaluation
module. The efficiency of the column is
determined in terms of height equivalent
to a theoretical plate (HETP), and the peak
asymmetry factor (A
s
). The result is logged
in the column logbook.
Column Performance Test
The column is filled with buffer solution.
Select inlet positions. Enter the solution
identity, volume, flow rate and incubation
time. By adding steps, several preparation
solutions can be used. Column Preparation
Packs AxiChrom™ columns, with a prede-
termined column type, by motorized power
or by a flow of hydraulic liquid that pushes
the adaptor down. The user initiates the
start of compression at the exact point
when the adapter reaches the consolidat-
ed bed surface. The adapter compresses
the bed according to the packing factor or
target bed height as selected. Two Column
Performance Test phases (upflow and/or
downflow) can be performed after the
AxiChrom column has been packed. Intelligent Packing
(only available for ÄKTA
avant 150)
454 ÄKTA avant User Manual 29035184 AE
9 Reference information
9.6 Predefined methods and phases