GE AKTA avant, Руководство пользователя

Страница: 452 / 538

Included phases Principle Predefined purification
method
After equilibration and sample application
(use a buffer containing a high salt concen-
tration, e.g, 2 M Ammonium Sulphate) hy-
drophobic proteins are adsorbed to the
column ligand. After a wash to remove
unbound sample, elution is performed us-
ing a gradient of decreasing salt concen-
tration. Then, the column is washed and
re-equilibrated with start buffer. After col-
umn re-equilibration, a fraction collector
accumulator wash is done to minimize the
risk for salt precipitation. Hydrophobic Interaction
Chromatography (HIC)
The sample application loops of the loop
valve are manually filled with samples. The
method will guide the user through the
process by pausing and displaying on-
screen instructions. Up to five loops can
be filled with different samples. The loops
are filled in descending order. Partial or
complete loop fill can be chosen. Automat-
ic washing of loops and flow path are inte-
grated in the method. Manual Loop Fill
The required components for this phase
are a column valve and a loop valve.
452 ÄKTA avant User Manual 29035184 AE
9 Reference information
9.6 Predefined methods and phases