4.5.1
Prime buffer inlets and purge system pumps
Overview
The procedure consists of the following stages:
Description
Stage
Prime all inlet tubing to be used during the run.
1
Validate priming of inlet tubing.
2
Purge System Pump B if pressure signal indicates air bubbles.
3
Validate purge of System Pump B.
4
Purge System Pump A if pressure signal indicates air bubbles.
5
Validate purge of System Pump A.
6
End the run.
7
To increase life length of the pump sealing rings, make sure that the pump
rinsing system is filled with fresh rinsing solution.
Note:
The procedures for purging the pump heads and priming the inlets using the
Process Picture, are described in the following topic. It is also possible to
perform the procedures from the Manual instructions dialog box.
Tip:
Prime inlet tubing
Follow the instructions to fill all A and B inlet tubing to be used in the run with appropriate
buffer/solution.
Action
Step
Make sure that all inlet tubing that is to be used during the method run is
placed in the correct buffer.
1
Open the System Control module.
2
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ÄKTA avant Operating Instructions 29101556 AB
4 Installation
4.5 Prime inlets and purge pump heads
4.5.1 Prime buffer inlets and purge system pumps