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8.2 Applying the sample
Introduction
A number of different sample application techniques are available. The sample can be
applied either directly onto the column using the Sample pump, or via a loop. A loop can
be filled either manually or by use of the Sample pump. This section describes sample
application using a syringe to manually fill a capillary loop. The two stages of the sample
application are described in the table below. For detailed instructions and information
regarding the different sample applications techniques, see User Manual.
Description Stage
The capillary loop is filled with sample. Load
The sample is injected onto the column. Inject
Sample application through a
capillary loop
A capillary loop is manually filled with sample using a syringe connected to the Injection
valve port Syr. During the method run, the sample is automatically injected onto the
column. The loop is emptied and washed out using buffer from the system pumps. The
total buffer volume to be used for emptying and washing the capillary loop is set in the
Phase Properties tab of the Sample Application phase at Empty loop with.
98 Getting Started with ÄKTAavant and UNICORN 6 28-9440-69 AB
8 Run a method
8.2 Applying the sample