Biacore T100 Software Handbook BR-1006-48 Edition AE
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Concentration analysis 8
provided that the report point used for the calibration curve exists in the cycles
from the kinetic run, calculated concentrations will be reported for these
samples.
In order to ensure that concentration analysis is correctly evaluated in
combined result sets, make sure that all files that contribute to the combined
result set are derived from concentration analysis runs. Provided that each file
starts with a calibration curve that is not excluded from the evaluation, the
results will be calculated within the respective files even in the combined result
set. However, if calibration curves at the beginning of files are missing or
excluded, there will be overlap between the individual file sets and some
samples from one file will be evaluated on the basis of calibration curves from
another file. In such cases, it is important to ensure that the calibration curves
and sample analyses in the different files refer to the same analyte and are
performed under as far as possible identical condition.
8.3
Evaluating calibration-free measurements
Calibration-free concentration analysis calculates the analyte concentration
from the measured mass transport properties and values for the diffusion
coefficient and molecular weight, provided as evaluation variables when the
assay is run (Section B.2). The evaluation is based on fitting the sensorgram data
to a model of 1:1 interaction kinetics, with mass transport parameters
calculated from the supplied diffusion coefficient and molecular weight, and
with the analyte concentration set as a globally fitted variable.
To evaluate calibration-free concentration analysis, choose
Concentration
Analysis:Calibration-free
from the evaluation toolbar, or choose
Add
Concentration Analysis:Calibration-free
from the
Evaluation
menu.
Содержание Biacore T100
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