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X-Cite® mini+ User Guide
035-00587R rev. 5
6.3
Low Illumination Intensity
1.
SpeedDIAL Setting Check:
a.
Verify that the LED intensity is set to a sufficient level.
b.
Verify that the LED Head is turning on.
2.
Microscope Check:
a.
Verify that the Microscope Flange is appropriate for the microscope configuration.
Note: Some flanges have the same mechanical fitting but different optics.
b.
Verify that everything in the microscope beam path is properly aligned and open, e.g.
shutters, apertures, diaphragms, filters, filter cubes, etc.
c.
Verify that microscope filter sets are for the appropriate wavelengths.
d.
Verify that air objectives are clean / immersion objectives have enough of the
appropriate fluid.
6.4
Other Potential Symptoms & Questions
Category
Symptom
Action
speedDIAL
Favorite intensity setting: double-
click turns off LED instead of going
to the stored setting
Increase delay in the speed (Spd) setting.
New settings are forgotten when
unit is shut down and powered up
After changing settings, wait at least five
(5) minutes before shutting unit down.
Turning dial does not scroll through
the menu options
Turn dial in the opposite direction.
“Lock” icon is on display, speedDIAL
not responding to manual control
Send “unlock” command via computer OR
power down unit and restart.
TTL Triggering
TTL signal does not trigger unit
Enable TTL mode.
TTL response time is longer than
usual
Verify that timeout settings are
appropriately set.
Audible Noise
miniCUBE fan never turns off, even
when LED is not on
Fan continues to run for five (5) minutes
after LED is turned off. If it runs longer,
disable TTL mode (it is likely enabled).
UV Excitation
No DAPI signal is visible, all other
fluorophores are fine.
Unit is in “UV Off” mode. Use speedDIAL
to enable “UV On” mode.
USB
Communication
Computer does not detect X-Cite
unit via USB connection
Power down X-Cite unit. Wait at least 10
seconds. Restart.
Note: The LED head has an additional feature of a temperature-controlled fan which only turns on when
required by the unit, thereby preventing additional vibration or acoustic noise which may interfere with
sensitive applications. The fan in the LED head will automatically turn on when the LED temperature
reaches 55°C and turn off when the LED temperature is below 45°C. Timing will vary depending on the
ambient temperature of the LED head’s location and duty cycle.
