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Endress+Hauser Analytik Jena InnuPure C16 touch, Руководство по эксплуатации, Страница: 18 / 69

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Function and setup 

InnuPure C16 

touch

 

18 

3.4

 

Design of the InnuPure C16 touch  

The InnuPure C16 

touch

 is a stand-alone device. The device is equipped with a com-

bined magnet/heating unit. This allows for the nucleic acids that bind to magnetic and 

paramagnetic particles to be effectively collected at the cavity floor while the washing 

solution, for example, evaporates at temperatures of up to 70 °C. The device is oper-
ated and controlled via a 10” tablet attached to the front and the IP

extract

 program. 

Sample processing follows pre-installed protocols tailored to the extraction kits. The 

kits include all reagents necessary for extraction, permitting extraction to run fully au-
tomated. 

 

Fig. 2 

Front view of the InnuPure C16 touch 

1  Tablet with IP

extract

 control program 

2  LED status indication 

3  Upper front door, manual opening for cleaning 
4  Lower front door, automatic opening for insertion 

of the sample tray 

Содержание Analytik Jena InnuPure C16 touch

Страница 1: ...Operating manual InnuPure C16 touch Extraction System...

Страница 2: ...Fax 49 3641 77 9279 Email bio application analytik jena de http www analytik jena com Microsoft and Windows are registered trademarks of Microsoft Corp InnuPure and TD Technology are trademarks of An...

Страница 3: ...Space requirement 21 4 2 Connections and control elements 22 4 3 Connecting the InnuPure C16 touch and switching it on off 24 5 Sample preparation 26 5 1 Preparing buffers and solutions 26 5 2 Prepar...

Страница 4: ...d care 55 7 1 Cleaning the InnuPure C16 touch 55 7 2 Exchanging fuses 57 8 Fault removal 58 9 Transport and storage 59 9 1 Transport 59 9 1 1 Preparing the device for transport 59 9 1 2 Transport note...

Страница 5: ...ned in action units Warnings are indicated by warning triangles and a signal words The type and source of the danger are stated together with notes on preventing the danger The meaning of the signal w...

Страница 6: ...terms and conditions of Analytik Jena Deviations from the intended use described in this user manual result in limitations of warranty and liability in the event of a damage Damage to wearing parts is...

Страница 7: ...ructions in this user manual and the local safety regulations that apply to the operation of the device the general applicable regulations regarding acci dent prevention occupational health and safety...

Страница 8: ...echnical condition The InnuPure C16 touch corresponds in its design and construction with the current state of the art technology Unauthorized modifications or changes especially such that affect the...

Страница 9: ...values and statutory limits for lifting and carrying loads without auxiliary equipment must be observed and adhered to 2 6 Safety instructions operation 2 6 1 General The device may only be operated i...

Страница 10: ...onnector must be disconnected from the mains outlet Any work on the electronics behind the device enclosure may only be carried out by the customer service of Analytik Jena and specially authorized te...

Страница 11: ...le if the filter tips are used Reagent cups PP or PTFE The above mentioned components are not resistant to the following substances hydrogen fluoride HF hydrofluoric acid highly concentrated acids cle...

Страница 12: ...e isolated adsorb at surface functionalized magnetic or paramag netic particles The necessary extraction chemicals are added manually as necessary and enable the purification of ultra pure nucleic aci...

Страница 13: ...e manual for the respective extraction kit Mixing lysate and magnetic particles Transferring the binding buffer to the sample Mixing binding buffer and sample Collecting magnetic particles and transfe...

Страница 14: ...o their origi nal holding position Washing the tips To prevent the carry over of washing solution resi due the tips used are flushed between specific wash ing steps Aspirate washing solution from the...

Страница 15: ...uate transfer The nucleic acids are now extracted are transferred into the elution tube and are available for further downstream applications Note Filling levels and filling colors are only shown for...

Страница 16: ...acids are released from the cells during lysis Transferring the binding buffer to the sample Binding Transfer of the binding buffer After lysis the binding buffer is transferred to the lysed samples T...

Страница 17: ...tion protocol and is defined at the start of the protocol The correspondingly selected volume is removed from the holding position and transferred to the elu tion position Elution During the elution p...

Страница 18: ...e evaporates at temperatures of up to 70 C The device is oper ated and controlled via a 10 tablet attached to the front and the IPextract program Sample processing follows pre installed protocols tail...

Страница 19: ...ed onto the sample tray Depending on the number of samples reagent plates deep well plates with eight sample positions or reagent strips for individual samples will be used Thus within a single extrac...

Страница 20: ...ypical Windows functions with a clearly structured menu interface Thus the entire system becomes a stand alone device IPextract contains pre installed extraction and decontamination protocols Thus the...

Страница 21: ...h must only be connected to a properly grounded mains outlet in accordance with the voltage specifications on the rating plate The InnuPure C16 touch is operated on single phase alternating current gr...

Страница 22: ...nnections and control elements The mains connection the fuse holder and the mains switch are located at the rear side of the InnuPure C16 touch Fig 7 Rear connections and switches on the InnuPure C16...

Страница 23: ...trol the InnuPure C16 touch The tablet is attached via a support bracket to the basic device and can be easily mounted and dismounted The tablet features an interface with the basic device and two USB...

Страница 24: ...e C16 touch Before that remove the protective cap from the inter face on the device and store for later use Place the tablet with support bracket on top of the de vice inserting the two guide pins see...

Страница 25: ...the tray via the button Wait until the drawer with the sample tray is completely ejected 2 Remove the sample tray Remove used reagent structures and elution tubes Clean the sample tray if required 3...

Страница 26: ...rding to the descriptions in the corre sponding kit manuals Follow the instructions described in the preparatory steps within the kit components of the kit manual 5 2 Preparing the sample tray for kit...

Страница 27: ...Here too the red marking on the adapter must point to the red point on the sample block Attention Load both sample blocks of the sample tray evenly see be low 4 Insert a maximum of 4 strips into the...

Страница 28: ...gh the sealing film and create large open ings 9 Make sure that all cavities are opened completely at least 7 mm diameter After extraction Close the elution tubes using lids For an extraction run it i...

Страница 29: ...extraction the sample tray must be loaded with the reagent plates and or rea gent strips of the kit used In the example the sample tray is populated with a reagent plate and four reagent strips When...

Страница 30: ...he plates and strips from being pulled out of the sample blocks during the extraction process 6 Insert the filter tips and the elution tubes into the tip block Insert the SmartExtraction filter tips w...

Страница 31: ...ll not be filled With the strips make sure that for every strip the tips and the elution tube are in the corresponding positions in the tip block arrows in figure on the left 8 Open all cavities of th...

Страница 32: ...y extracting one sample insert an adapter with an empty strip or an empty deep well plate into the second sample block of the sample tray If processing more than one reagent strip during the extractio...

Страница 33: ...traction and saving the process log file in the project PROJECTS Opening a stored project of a completed extraction run OPTIONS Checking and executing individual device functions with special tools Se...

Страница 34: ...Risk of data loss The tablet does not have its own battery When you turn off the basic device using the mains switch you switch off the tablet at the same time The tablet is not intended for any other...

Страница 35: ...are displayed separately 1 Open the HOME start page 2 Select the menu item TEMPLATES LAST TEMPLATES 3 Select the template in the menu The template is loaded 6 3 Editing and saving extraction parameter...

Страница 36: ...late screen Display of the selected page 4 Icon bar Function icons The functions and icons vary depending on the contents of the selected screen 6 3 1 The General page entering general extraction run...

Страница 37: ...the corresponding kit information To switch between these two options tap on the options button The dot is on the side of the method that has been selected The selected method determines which kits ar...

Страница 38: ...e to use several kits in one extraction run if these kits use the same extraction protocol The protocol of the first kit is used for the extraction run Details on the other kits is stored as infor mat...

Страница 39: ...nt Description KIT NAME Name of the kit adopted from the KIT INFORMATION page LYSIS TYPE Select lysis type depending on the source material INTERNAL LYSIS Lysis is processed inside the device EXTERNAL...

Страница 40: ...ple types 1 Select the loading of the sample tray side PLATE reagent plate or STRIP reagent strip 2 Tap on a white sample position field The field is highlighted and marked with a red frame 3 Tap on T...

Страница 41: ...of the table The upper dot represents the left block of the sample tray the lower dot represents the right block One table row is assigned to each location in the sample block 1 Swipe vertically over...

Страница 42: ...3 Enter the file name in the SAVE AS field 4 Tap on The layout data is now exported and can be edited in Excel 1 Tap on on the SAMPLE LAYOUT template page 2 Select the storage location Tablet PC or U...

Страница 43: ...u can create as many subfolders as you want using the DATA function 1 Tap on after having entered all parameters on the TEMPLATE page 2 Select the storage medium TABLET PC or USB MEMORY STICK 3 Enter...

Страница 44: ...not touch the tray Do not reach inside the InnuPure C16 touch The extraction starts During the run the currently performed steps are shown in a video sequence In addition the following time related in...

Страница 45: ...locked This helps for example to avoid interruption of an ongoing extraction process due to accidental touchscreen operation 1 Tap on the padlock item in the upper right corner of the touchscreen The...

Страница 46: ...mplate information can be saved Storing log data of the extraction run in a project p 45 If the project is loaded the extraction parameters are shown on the pages of the PROJECT window The page MONITO...

Страница 47: ...in the SAVE AS field 4 Tap on The template information will be exported in a PDF document A project is closed after completion of the extraction run In order to use the parame ters of the protocol for...

Страница 48: ...st a defined temperature is set and held for a pe riod The setting of the temperature and the temperature constant during the holding time are documented The log file of the test will be saved in the...

Страница 49: ...ion for the program display Currently English is the only language available Selection of display of extraction protocols Display of the program version Software update Managing user defined sample ti...

Страница 50: ...PTIONS GENERAL from the HOME page 2 Tap on beside VERSION INFO The version number of the actual IPextract version is displayed 6 7 4 Updating IPextract The update of IPextract can be started on page G...

Страница 51: ...n near SAMPLE TYPE MANAGEMENT The SAMPLE TYPE MANAGEMENT page appears with the user defined sample types Fig 22 SAMPLE TYPE MANAGEMENT window Following functions are available in the SAMPLE TYPE MANAG...

Страница 52: ...system The tablet has no active antivirus protection The user is responsible for the safety of the USB Memory Stick The IPextract program features a data management that supports the exchange of data...

Страница 53: ...USB Memory Stick as follows 1 Tap on the file to be exchanged The highlighted file is marked with a red bar 2 Copy it to the clipboard with or cut it with 3 Tap on the target folder 4 Paste the file i...

Страница 54: ...file 2 Tap on 3 Enter the new name 4 Tap on The folder or file is now displayed with the new name 1 Tap on the folder or file 2 Tap on 3 Confirm the prompt about deleting the folder file with The fol...

Страница 55: ...r to start cleaning the interior of the InnuPure C16 touch After this cooling down period the temperature directly below the heating strip is less than 50 C Avoid contamination by handling sample subs...

Страница 56: ...using a spray head first apply the disinfection agent on suitable cloths Only the use of ethanol or isopropanol containing disinfection agents are approved for wiping disinfection The use of disinfec...

Страница 57: ...t the mains plug from the connection at the rear of the device 2 Carefully open the fuse holder using a screwdriver 3 Replace the defective mains fuses Use 2 x 4A 250 V fuses only 4 Close the fuse hol...

Страница 58: ...result 2 If tips with or without liquid are still attached to the cones after a protocol abor tion or power failure the tips are ejected via a safety function starting automati cally the next time a...

Страница 59: ...e InnuPure C16 touch two persons are required to lift the device by holding onto the bottom of the device ATTENTION Use the transport packaging recommended by AJ for packaging Only this provides op ti...

Страница 60: ...ample chamber 1 Remove remaining consumables from the InnuPure C16 touch 2 Move the dispensing unit with the wiper in the top position 3 Shut down the tablet and switch off the basic device Dis connec...

Страница 61: ...lace the in sert in the priming station 8 Place the power cable in the insert 9 Mount two side panels on the pallet 10 Place the foam frame for the device base onto the pallet 11 Wrap the InnuPure C16...

Страница 62: ...and slide it in the compartment in the upper foam frame 15 Place the accessory box between the struts of the foam frame 16 Cover the accessory box with foam foil 17 Place the cardboard cover on the f...

Страница 63: ...required to lift and carry the device When moving the InnuPure C16 touch in the laboratory observe the following 1 Switch off the tablet and the InnuPure C16 touch Remove the tablet from the de vice 2...

Страница 64: ...The atmosphere must be low in dust and free from aggressive vapors If the device is not installed immediately after delivery or not required for prolonged periods it should be stored in its original p...

Страница 65: ...C16 touch Disposal 65 10 Disposal At the end of its service life the InnuPure C16 touch and all its electronic components must be disposed of in accordance with the applicable regulations as electron...

Страница 66: ...teel pistons Cleaning System components are easily accessible through large split front door Temperature control Heating up to 90 C Application parameters Consumables Completely contained in the requi...

Страница 67: ...8 positions in parallel on the strips or plates Adapter for reagent strips optional Adapter for accepting a maximum of 4 reagent strips Barcode reader optional Sample name detection via barcode readin...

Страница 68: ...requirements of EC directives 2014 35 EU and 2014 30 EU The device leaves the manufacturer in a pristine and technically safe state To maintain this condition and to ensure safe operation the operator...

Страница 69: ...InnuPure C16 touch EC Declaration of Conformity 69 12 EC Declaration of Conformity...

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