Biometra TAdvanced
Creating, editing and saving programs
Version 02_20
49
7.6
Editing steps
When editing program steps, the following parameters can be entered in the Biometra
TAdvanced software:
Parameter
Description and limits
T
EMPERATURE
Temperature for a step in °C. The target temperature can be set between 3.0°C and 99.0°C in
tenths of a degree.
H
OLD TIME
Hold time for a step in h:mm:ss. Values between 0 and 9 hours, 0 and 59 minutes and 0 to
59 seconds can be programmed.
To program a pause and set the hold time to infinite, enter ∞ in one of the input fields for
h:mm:ss.
G
O
T
O
Defines the step number to which the program returns.
C
YCLES
Defines the number of repetitions. The maximum number of repetitions is 999.
Temperature increment by which the target temperature is increased or decreased with each
cycle. The temperature increment can be ± 20.0°C.
Time increment (e.g., for long range PCR) by which the time is increased with each cycle. The
time increment can be between 0s and 240s.
Average heating and cooling rate between steps. The heating and cooling rate can be set
from 0.1°C/s to max. in tenths of a degree. (The maximum ramp rate is sample bock
specific). The ramp rate specifies the speed at which the selected step is reached with.
It is possible that in some models this display may show a slightly higher maximum ramping
rate than is achieved according to the technical specifications of the device. This is technically
justified and achieves optimum control of the maximum value. In fact, the maximum heating
and cooling rate is achieved as specified in the technical specifications.
Establishes a temperature gradient across the sample block. The maximum gradient
temperature range and the temperature range that can be used for the gradient will depend
on the installed sample block.
The Biometra TAdvanced offers two options for editing a step in a PCR program:
Change all parameters for a step on a screen
Change individual parameters directly in the spreadsheet or graphical display
7.6.1
Editing all parameters of a step
1.
Activate a step in the spreadsheet or graphical view (see section "Programming
modes" p. 44).
2.
To edit the activated step, press the [E
DIT
] button.
E
DIT
In the graphical view you can also access the screen for editing a program step by
touching the currently activated step again.