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Zoë-CM2

User Guide

Содержание Zoe-CM2

Страница 1: ...Zo CM2TM User Guide...

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Страница 3: ...oth the top and bottom channels of Organ Chips It also allows users to determine stretch parameters including frequency and amplitude of the Organ Chip membrane What s more the Zo CM2 Culture Module h...

Страница 4: ...Installation Introduction Specifications Safety Page 7 Page 17 Page 21 Zo CM2 Culture Module Table of Contents Introduction Page 13 1 2 3 4 Zo Culture Module Table of Contents...

Страница 5: ...Page 5 5 Use Page 45 Page 49 Page 53 Use Care Settings Help Support Page 55 6 8 9 Page 29 Updating Firmware Downloading Logs 7...

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Страница 7: ...iew of Zo and its functions Equipment what s in the box 1 Zo CM2 Culture Module 2 Chip Cradles 3 Gas Vacuum Supply Line 4 Zo Core Update Cable 5 SensorPush HT1 Temperature and Humidity Smart Sensor 6...

Страница 8: ...igation and adjusting values Dial Button Used to confirm selections parameters and other edits to system settings Display Shows the current Zo parameters displays system status and provides alerts and...

Страница 9: ...1 Bottom Channel Inlet Port 2 Top Channel Inlet Port 3 Vacuum Ports 4 Top Channel Indicator 5 Top Channel Outlet Port 6 Bottom Channel Outlet Port 7 Chip Body 8 Top Channel Indicator Organ Chip Config...

Страница 10: ...1 Pod Reservoir Lid 2 Pod Reservoir 3 Organ Chip 4 Chip Carrier Pod Configuration Zo CM2 Culture Module Introduction...

Страница 11: ...Page 11 1 Top Channel In 2 Top Channel Out 3 Vacuum Chamber 4 Bottom Channel Out 5 Bottom Channel In Pod Components...

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Страница 13: ...Page 13 Specifications Page 13 2 This section provides specific technical information and operational requirements Included are system dimensions thermal and gas requirements and modes of operation...

Страница 14: ...kW day Capacity 12 Pods Operating Instrument Rating 24 VDC 3 5 A 50 60 Hz Requirements Gas Input Pressure 276 kPa 35 40 psig 5 Gas Input Composition 5 CO2 balance air Vacuum Input Pressure 70 kPa 10...

Страница 15: ...a Ethernet Description Basic Functionality Zo CM2 is not connected to the internet Zo CM2 can only be operated using the screen on the instrument Zo CM2 has access to the internet Zo CM2 can be operat...

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Страница 17: ...fety Page 17 3 This section includes recommended precautions when handling and using Zo CM2 The information here describes how users can minimize the chance of harming themselves or the module during...

Страница 18: ...nstructions in the user guides Regulatory Compliance and Testing This product has been tested to the requirements of 61010 1 IEC EN UL CSA IEC 61010 2 10 Safety Requirements for Electrical Equipment f...

Страница 19: ...logical material Service and maintenance should only be completed by Emulate certified personnel Never attempt to disassemble or repair Zo without assistance Please be considerate of the instrument te...

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Страница 21: ...n covers the basic installation of Zo CM2 Please reach out to Emulate Field Support for detailed installation guidance and support if needed prior to proceeding with installation Ensure Orb and all hu...

Страница 22: ...Ethernet Cable Power Cord Zo CM2 5 1 2 3 4 Power Supply Zo CM2 Items for Installation Zo CM2 comes with the following items for installation Gas Vacuum Supply Line Zo CM2 Culture Module Installation...

Страница 23: ...3 Incubator 100 CO2 Gas Supply 6 7 8 Orb HM1 Zo CM2 Items for Installation The user will need to provide the following equipment for installation Note Contact Emulate for guidance on user provided equ...

Страница 24: ...Smart Sensor from the Zo CM2 box This will be used to check the incubator temperature prior to Zo CM2 installation 5 To set up SensorPush HT1 a Download the SensorPush app to a smartphone available on...

Страница 25: ...mperature and humidity settings a Open the SensorPush app and monitor the temperature over time b Wait until the temperature of the incubator is stable and is within the acceptable range of 36 C to 38...

Страница 26: ...Connect the Ethernet Cable to an active network port and run it through the incubator access hole 4 Connect the Gas and Vacuum Supply Line Power Cord and Ethernet Cable to the Zo Bulkhead 5 Connect th...

Страница 27: ...ort on the back of Zo 1 To connect to a wired network plug the supplied Ethernet Cable into the network port on Zo and connect the other end to an active ethernet port 2 The system will connect automa...

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Страница 29: ...Page 29 Use Page 29 5 This section describes proper interaction with and operation of Zo CM2 The instrument should always be used in an incubator while following aseptic procedures...

Страница 30: ...c Dial button d Bay Activation Buttons e Power Button Powering On To power Zo on push the Power Button located on the upper right corner of the faceplate Zo will then go through its startup routine u...

Страница 31: ...when a chip is connected to the Pod 1 Prepare Pods per desired organ specific protocol 2 Insert Tray with up to six Pods into a Zo Bay 3 Repeat Step 2 in the opposite Bay if priming more than six Pod...

Страница 32: ...ppear to show the progress of the Prime Cycle Running the Prime Cycle Continued 6 Press the Dial Button to select Start which will initiate the Prime Cycle This will cause the manifolds to lower Zo CM...

Страница 33: ...nd timer will disappear the manifolds will rise and the Bay s will be deactivated Zo CM2 Use 9 Remove the primed Pods from Zo and attach the prepared chips to the Pods Reference organ specific protoco...

Страница 34: ...edia Type 1 Using the Dial hover over the areas below Media denoting the Top and Bottom channels 2 Press the Dial Button to edit the Media type Rotate the Dial to select between Fluid and Air Gel Zo C...

Страница 35: ...cting Media Type Continued 3 After hovering over the desired media type press the Dial Button to confirm a Note If selecting Air Gel the flow rate will automatically be set to 0 to prevent media flow...

Страница 36: ...e 1 Using the Dial hover over the areas below Rate denoting the Top and Bottom channels 2 Press the Dial Button to begin editing flow rate Rotate the Dial to adjust the flow rate between 10 and 1 000...

Страница 37: ...Page 37 Zo CM2 Use Programming Flow Rate Continued 3 After rotating to the desired flow rate press the Dial Button to confirm 4 These settings will now take effect on Zo...

Страница 38: ...g the Dial hover over the Strain parameter This option allows you to set the amount of stretch on the chips 2 Press the Dial Button to begin editing the strain Rotate the Dial to adjust the level of s...

Страница 39: ...gramming Stretch Parameters Continued 3 After reaching the desired level of strain press the Dial Button to confirm 4 Using the Dial hover over the Frequency parameter This option allows you to set th...

Страница 40: ...meters Continued 5 Press the Dial Button to begin editing the frequency Rotate the Dial to adjust the frequency between 0 00 and 0 40 Hz 6 After reaching the desired frequency press the Dial Button to...

Страница 41: ...Cycle This ensures that Regulate will run properly and will not disrupt gel or ALI conditions To avoid drying out the reservoir during the Regulate Cycle ensure there is sufficient media in all pods...

Страница 42: ...option Press the Dial Button to select Start initiating the Regulate Cycle 4 A status bar and timer will appear and show progress of the Regulate Cycle To cancel the Regulate Cycle rotate the Dial to...

Страница 43: ...Regulate Cycle Continued 5 Once the Regulate Cycle is complete the status bar and timer will disappear and the system will revert to the preset flow and stretch parameters Shutting Down Press the Powe...

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Страница 45: ...Page 45 Settings Page 45 6...

Страница 46: ...ls on Zo Model Name ID Serial Number Software Version and Hardware ID This screen can be accessed by hovering over and selecting the icon on the lower right of the screen To close the System Informati...

Страница 47: ...icon in the top bar of the display Press the Dial Button to bring up the Network Connection screen If there is an active ethernet connection Connected will automatically be displayed along with the IP...

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Страница 49: ...Page 49 Updating Firmware Downloading Logs Page 49 7...

Страница 50: ...emulatebio com utility hub The application is compatible with computers running Windows 7 or a later version 1 Once Utility Hub has been downloaded open the application and select Update Firmware 2 F...

Страница 51: ...Page 51 Zo CM2 Updating Firmware Downloading Logs Downloading Logs 1 Open Utility Hub and select Download Logs 2 Follow the instructions displayed on Utility Hub to download the logs from Zo CM2...

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Страница 53: ...age 53 Help Support Page 53 8 Contact Emulate Support for any issues with Zo CM2 Email support emulatebio com Website https emulatebio com contact support Phone 1 844 902 4477 Toll Free 1 781 583 3515...

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Страница 55: ...Page 55 Care Page 55 9...

Страница 56: ...an appointment Regular checks and recordkeeping provide helpful information should any troubleshooting be required Zo does not require significant maintenance However it is necessary to clean the exte...

Страница 57: ...Confirm the Power Cord is fully connected to rear of Zo Culture Module Contact Emulate for troubleshooting Replace Power Cord contact Emulate for replacement Insert Tray until the Tray snaps into pla...

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