Dionex UVD 170S, Руководство по эксплуатации

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Operating Instructions UVD 170S/340S
14
Problem Probable cause Remedial action
Strong baseline drift Insufficient warm-up time Allow min. 30 min. warm-
up
Column contaminated Rinse or replace column
System not equilibrated Continue to rinse system
until equilibrated
Unstable environment Ensure constant temperature
and humidity
Solvent contaminated Change solvent
Flow cell contaminated
Clean flow cell (
→
sec.
6.3.4)
Lamp defective Replace lamp
Solvent reservoir is placed too
low Place reservoir on the same
level as flow cell
High noise level Solvent contaminated Replace solvent
Lamp aged Replace lamp
Gas bubbles in system Prime system
Pressure pulsation from pump Check and prime pump
Solvent reservoir is placed on
the floor Place reservoir on the same
level as flow cell
Wrong wavelength Select suitable wavelength
Connecting cable between
UVD 170S/340S and PC not
correctly installed Connectors on the detector
and the PC must be inserted
evenly and locked into place
with latching devices on both
sides.
Peak broadening Capillaries too long or has
excessive internal diameter Shorten or replace for
narrower capillaries
Column overloaded,
contaminated or aged
Rinse or replace column
Solvent aging Replace with fresh solvent
Analysis is not
reproducible Sample is unstable Use fresh sample or alter
conditions
Irreproducible gradient Change gradient program /
check pump
Unstable environment Ensure constant temperature
and humidity
Column overloaded,
contaminated or aged
Rinse or replace column
CHROMELEON
TM
Error Messages
A list of CHROMELEON
TM
error messages is included in the CHROMELEON
TM
Installation
program on the tab dialog box "Error Levels". See also section 4.3.3.