Dionex UltiMate 3000 Series, Руководство по эксплуатации

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UltiMate 3000 Series:
SD, RS, and BM Pumps
Operating Instructions Page 211
12.3 Properties of Common Solvents
The table summarizes the properties of the most important solvents in HPLC [1, 2]
Acetronitrile Dichloromethane n-
H
exane
Isorpopanol Met
h
anol
Tet
rahydrof
u
rane
Water
UV Transmission at [nm]
20% (0.7 AU)
80% (0.1 AU)
98% (0.01 AU)
190
195
220
235
245
260
200
225
260
210
230
260
210
235
260
255
370
310
--
--
< 190
Refraction Index (RI) at 20 °C 1.344 1.424 1.376 1.378 1.329 1.406 1.333
Boiling Point (BP) in °C at 1013 hPa 82 40 69 82 65 66 100
Vapor Pressure (VP) at 25 °C 118 582 202 60 169 216 32
Viscosity (

) at 20 °C (cP = mPa*s)
0.37 0.44 0.33 2.3 0.60 0.55 1.00
Density (

) (g/mL) 0.78 1.32 0.66 0.78 0.79 0.88 0.997

/

(cP*mL/g)
0.47 0.33 0.50 2.9 0.76 0.62 1.00
Compressibility (

) at 20 °C (Mbar
-1
)
99 97 160 100 123 93 46
Critical Flow F
c
(mL/min)
1)
13 9.4 14 83 21 18 28
Linear Drop in Pressure
Δ p/l (MPa/m)
2)
0.06 0.08 0.06 0.40 0.10 0.10 0.17
Polarity (P')
3)
5.8 3.1 0.1 3.9 5.1 4.0 10.2
1)
F
C
= critical flow for 0.25 mm I.D. tubing
F
C
(mL/min) = 113 x 0.25 mm x

(cP) /

(g/mL)
Fc is an example of a hydrodynamic calculation.
2)
Δ p/l = linear drop in pressure for 1 mL/min and 0.25 mm I.D. tubing
Δ p/l (MPa/m) = 6.8 x 10
-6
x 1 mL/min x 100 cm x

(cP) / (0.25 mm)
4
Δ p/l is an example of a hydrodynamic calculation.
3)
P' is the polarity calculated by L.R. Snyder [3] from experimental measurements by L. Rohrschneider [4]
References
[1] K.K. Unger, E. Weber (Hrsg.), Handbuch der HPLC , GIT Verlag, 1995
[2] D.R. Lide, Handbook of Chemistry and Physics , 79
th
Edition, CRC Press, 1998-1999
[3] L.R. Snyder, Journal of Chromatographic Sciences , 16, 223, 1978
[4] L. Rohrschneider, Analytical Chemistry , 45, 1241, 1973