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QUICK START GUIDE
Amersham ImageQuant
800
Turning system ON
1. Press the power button on the side of the Amersham™
ImageQuant™ instrument
2. Press the power button on the mini PC attached to the rear
of the system
Starting imaging process
1. After system initialization, enter password and click
Login
2. Confirm
CCD status
on the bottom left corner of the screen
is green
3. Choose detection method from the
Home
screen to begin
• Options:
Chemiluminescence
,
Colorimetric
, and
Fluorescence
Utilities
•
Scheduler
is a booking tool to reserve the imager for a
specific date and time
•
Image library
allows access to the default save location
where images are stored with options to view, copy/move, or
delete images
• Use
Settings
to view, set, and change system settings,
if needed
Using the chemiluminescence
imaging mode
1. Under
Select Exposure
, choose settings (or choose
Open
method
if you have saved a method previously)
• Auto:
Sets exposure time automatically for the entire
blot based on highest pixel intensity. There is an option to
choose
Pre-capture
to select an area of interest, which
calculates optimal exposure time for the selected area.
• Manual:
Enter exposure time manually
• Time series:
Perform a series of exposures (maximum of
50). Signal is cumulated for each image if
Cumulative
is ON.
Toggle
Cumulative
OFF if summed images are not needed.
2. Click
Start
to begin imaging
Note
Use the black tray with white insert if using for colorimetric marker.
Chemiluminescence
detection is available in both upper and lower tray positions.
