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5.3 Image capture
About this section
This section provides simple instructions for capturing an image using the Amersham
ImageQuant 800 control software interface. For a more detailed description of indi-
vidual image capture methods, their features, and how to use them, see the Amersham
ImageQuant 800 User Manual (29383813).
Capture methods
The Amersham ImageQuant 800 supports three modes of image capture: chemilumi-
nescence, colorimetric, and fluorescence. The main functions and uses of these modes
are described in the table below.
Image capture
mode Imaging options Sample type
Chemilumines-
cence Chemilumines-
cence Enzymatically labelled membranes (e.g.,
horseradish peroxidase)
With colorimetric
marker Colorimetric markers (e.g., Amersham
Rainbow™ Markers)
Colorimetric Gel documenta-
tion Colorimetric stained gels (e.g.,
Coomassie and silver staining)
OD measurement Quantitative densiometry measurement
Fluorescence Fluorescence Fluorescent labelled membranes
DNA gels (e.g., ethidium bromide and
alternatives staining)
With colorimetric
marker Colorimetric markers (e.g., Amersham
Rainbow Markers)
SNOW mode
The Signal to Noise Optimization Watch (SNOW) reduces noise and avoids saturation
of the image. Multiple exposures are captured and merged by averaging into a single
image, thus improving the signal-to-noise ratio. This setting can be used for chemilu-
minescence and fluorescence imaging methods. For more information, see the
Amersham ImageQuant 800 User Manual .
Capture an image
To capture an image, follow the steps below.
5 Operation
5.3 Image capture
86 Amersham ImageQuant 800 Operating Instructions 29372604 AC