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Regenova

®

A Bio 3D Printer

Start up Manual 

Ver.3

Feb.2016

●

This Manual is subject to change without notice.  

●

Information is as of Feb, 2016.

Cyfuse Biomedical K.K 

ICN Building-5A 2-27-17, Hongo, Bunkyo-ku, Tokyo, 113-0033, JAPAN

TEL

：

+81-3-4455-7872  E-mail

：

[email protected]

URL

：

http://www.cyfusebio.com

1

Содержание Regenova

Страница 1: ...3 Feb 2016 This Manual is subject to change without notice Information is as of Feb 2016 Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sa...

Страница 2: ...4 4 Setting up Cell Accumu 16 5 3D printing 20 6 After 3D printing 25 Maturation 1 Maturation 27 2 Removing tissue construct from Kenzan 28 FAQ 29 Advanced Operation 31 Cyfuse Biomedical K K ICN Build...

Страница 3: ...e for 9X9 Kenzan CY0 104 00 1 1 500 Kenzan Base Plate for 26X26 Kenzan CY0 105 00 1 1 500 Kenzan Container CY0 103 00 1 500 Nozzle 25G CY0 107 00 60 units 250 Nozzle 26G CY0 102 00 5 Nozzle 27G CY0 10...

Страница 4: ...uantity 676 needles 26 x 26 Material Stainless Steel Heat resistant resin Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sales cyfusebm co...

Страница 5: ...mmended for regular spheroids Recommended single usage due to potential clogging Kenzan Base Plate For 9X9 CY0 104 00 For 26X26 CY0 105 00 Kenzan Container CY0 103 00 Air System CY0 101 00 Waste Bin C...

Страница 6: ...ulture enough cells for your 3D printing project It is recommended to use cells in near a log growth phase 6 Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3...

Страница 7: ...es cyfusebm com URL http www cyfusebio com Prior to 3D Printing 3 Designing your 3D structure 7 For more details please refer to the manual of B3D software Designs recommended for beginners Tube Use 9...

Страница 8: ...ding 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sales cyfusebm com URL http www cyfusebio com Prior to 3D Printing 4 Sterilizing Accessories and Tools Before sterilizati...

Страница 9: ...N TEL 81 3 4455 7872 E mail sales cyfusebm com URL http www cyfusebio com Prior to 3D Printing 4 Sterilizing Accessories and Tools For the nozzle and Kenzan it is recommended to prepare and autoclave...

Страница 10: ...it is recommended to sterilize them together in the following manner Air bubbles can disrupt camera images and cause failure to detect needle tips Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hongo B...

Страница 11: ...al K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sales cyfusebm com URL http www cyfusebio com 3D Printing 1 Powering on 1 START 2 STOP 3 ALARM STOP 4 ABNO...

Страница 12: ...ion power supply to ON and wait a minute until it is fully initialized 5 Power the PC on Stop alarm with ALARM STOP Main power operation power supply ON OFF Cyfuse Biomedical K K ICN Building 5A 2 27...

Страница 13: ...uidlines for Regenova The sash of the clean bench must be closed down to the bottom When an alarm is activated press ALARM STOP and ABNORMAL RESET Then operation can resume Buttons on the right tab of...

Страница 14: ...der Nozzle Holder Kenzan Container stage Air Tube Step Place the Waste Bin Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sales cyfusebm c...

Страница 15: ...sure to avoid touching the tip of the nozzle to keep it clean 3 Tighten the screw at the nozzle stand Step 6 Close the front panel of the clean bench down to the bottom and hold down Home Return 15 3...

Страница 16: ...Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sales cyfusebm com URL http www cyfusebio com Step 3 Inspecting Kenzan Make sure Home Return is pink If not hold down the button until it...

Страница 17: ...low Press total then the system will calculate how many layers are possible to us for the print Then press Apply Step 6 Adjust z direction interval 17 Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hon...

Страница 18: ...checks again to confirm successful pick up Press Inspection Parameters Select Type 1 or Type 2 tab Adjust set parameters or previous data can be inserted from File Parameters on Type 1 tab should be b...

Страница 19: ...dle Array Map page and adjust Ring Light of upper camera Press Apply to activate Recommended settings of lights for spot and ring light are as follows 9X9 standard around 100 9X9 tube around 80 26X26...

Страница 20: ...m 3D Printing 5 3D Printing 1 In the Needle Array Map page place 3 4 spheroids on the top layer 2 Press and hold START 3D printing only for those few spheroids 3 Press and hold STOP 3D printing after...

Страница 21: ...r with following steps Adjust diameter Uncheck capture image Press Execute Set Diameter to cover the diameter of the nozzle Adjust Threshold Uncheck Auto set Increase Threshold 10 Threshold column wil...

Страница 22: ...stop the alarm 3 Cover the Kenzan container to avoid contamination 4 Replace the current nozzle with a new one 5 Remove the cover from the Kenzan container and close the sash 6 Hold down Home Return a...

Страница 23: ...se Biomedical K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sales cyfusebm com URL http www cyfusebio com 3D Printing 5 3D Printing Troubleshooting 3 No or...

Страница 24: ...1 needle 1 and well 2 to start can be selected 24 Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo 113 0033 JAPAN TEL 81 3 4455 7872 E mail sales cyfusebm com URL http www cyfusebio...

Страница 25: ...OT2000 and then shut the PC down 25 To save all data File Save Experimental Parameters All This data can now be used for future printing by loading it from File Cyfuse Biomedical K K ICN Building 5A 2...

Страница 26: ...Turn off Operation power supply with the key ON OFF 1 Open the air bleed valve of the compressor by turning counter clockwise and drain water Typically Main Power remains ON Step 7 Cleaning Wash all a...

Страница 27: ...n Chamber Type 1 CY1 004 00 is strongly suggested Please refer to the following directions for using the Perfusion Chamber Type 1 27 Cyfuse Biomedical K K ICN Building 5A 2 27 17 Hongo Bunkyo ku Tokyo...

Страница 28: ...ce grid plate 2 on grid plate 1 Move both plates down to Kenzan base Maturation 2 Removing tissue construct from Kenzan Remove the construct after maturation on Kenzan Re insert the grid plate which w...

Страница 29: ...fter Needle Array Check alarm Check the selection of Kenzan Type page to ensure the correct sized Kenzan is chosen Stack S1 Bubbles are observed each time spheroids are skewered Check the Air System c...

Страница 30: ...not to contaminate the nozzle S13 Skewering to the wrong needle or place Press EMERGENCY STOP immediately There are several reasons for this Bubbles in the Kenzan Remove bubbles with pipette and see...

Страница 31: ...mirror to see the display Unlock You will only need to unlock once each time the machine is turned on 1 Hold down key until Loc on the display starts to blink 2 Press S Key firmly to unlock the displa...

Страница 32: ...ic needle to penetrate Press Setting 2 and point to the tip of the desired needle Then press Setting 2 again and press Apply 3 Then the nozzle will pick up and penetrate the spheroid onto the needle 3...

Страница 33: ......

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