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EHS1000 series

MANUAL

Rev. 44/2019

Gel Electrophoresis

Horizontal units

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Содержание EVS1100

Страница 1: ...EHS1000 series MANUAL Rev 44 2019 Gel Electrophoresis Horizontal units...

Страница 2: ......

Страница 3: ...th cm Sample Capacity Gel Volume 5mm thickness ml EHS1050 10 8 40 40 EVS1100 7 7 10 64 25 35 EHS1200 10 7 10 100 35 50 EHS1300 15 7 10 15 210 53 75 113 EHS1400 20 10 20 25 550 100 200 250 EHS1500 26 1...

Страница 4: ...s 2 Setting up the horizontal gel tank 2 Gel preparation 3 Gel pouring with model EHS1050 3 Gel pouring with casting dams 4 Gel pouring using the flexicaster 4 Gel pouring using tape method 5 Running...

Страница 5: ...ow quantities of various gases are produced at the electrodes The type of gas produced depends on the composition of the buffer employed To disperse these gases make sure that the apparatus is run in...

Страница 6: ...larity and the correct orientation of the cables black is negative and red positive Remove the lid from the unit Note if the lid is not removed fitting the cables may result in un tightening of the go...

Страница 7: ...ed This is best viewed against a light background Crystals appear as translucent crystals These will interfere with sample migration if not completely dissolved The gel must be cooled to between 50 C...

Страница 8: ...tip Allow the agarose to set ensuring that the gel remains undisturbed Carefully remove the gel casting gates and comb and transfer the gel including tray to the main tank Gel pouring using the flexi...

Страница 9: ...e fill the unit to 5 mm above the gel Load the samples into the wells using pipettes Multi channel pipettes can be used for loading samples with MultiPipette compatible combs see listing in accessorie...

Страница 10: ...89 mM tris pH 7 6 89 mM boric acid 2 mM EDTA 10 x 1l dissolve in 750 ml distilled water 108 g tris base FW 121 55 g boric acid FW 61 8 40 ml 0 5 M EDTA pH 8 0 Fill to 1 litre with distilled water Sam...

Страница 11: ...Samples do not run straight Comb may be warped and should be replaced Running tray may be warped and should be replaced Reduce the voltage Choose a buffer with suitable ionic strength and buffering ca...

Страница 12: ...slow migration Check enzyme activity as it may require longer digestion or different restriction buffer Prepare fresh sample if nuclease contamination is suspected Choose agarose with low endosmosis...

Страница 13: ...Horizontal Gel Electrophoresis units 9 Certificate CE...

Страница 14: ...ir or replace free of charge units where improper solutions or chemicals have been used For a list of these please see the Care and Maintenance subsection Consort products are for research use only Co...

Страница 15: ...Horizontal Gel Electrophoresis units 11...

Страница 16: ...l Gel Electrophoresis units 12 Consort bvba Hertenstraat 56 unit 9 2300 Turnhout Belgium Tel 32 0 14 41 12 79 Fax 32 0 14 42 91 79 Sales sales consort be Support support consort be Information info co...

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