Discover Bio
™
Manual Microwave Peptide Synthesizer
600423 • Revision 2 • August 2017
9
Recommended Synthesis Parameters
Recommended Microwave Methods
Recommended Reagents for 0.1 mmol Scale
Activator Base Solution
Prepare a solution of ~0.3 M DIEA in DMF.
Deprotection Solution
Prepare a solution of 20% piperidine in DMF with 0.1 M HOBt monohydrate.
Method
Power
Max Temp.
Run Time
Delta Temp.
Deprotection
20 W
75 °C
3:00
5 °C
Coupling
20 W
75 °C
5:00
5 °C
TFA Cleavage
15 W
38°C
30:00
5 °C
NOTE
Cysteine and histidine couplings should be performed at 50 °C to reduce racemization. Cleavage
temperatures should never exceed 43 °C. All values for Power are suggested, and should be
adjusted depending on reaction scale. The goal is to reach the maximum temperature in 75
seconds for Deprotection and Coupling, and in 120 seconds for Cleavage.
Number of
Couplings
0.1 mmol Scale
mL
DMF
mL
DIEA
Final
Vol.
5
19
1
20
10
38
2
40
Per Coupling:
4 mL
Final Volume
0.1 mmol Scale
mL
DMF
mL
Pip.
g HOBt
monohydrate
50
40
10
0.77
100
80
20
1.5
500
400
100
7.7
Per Coupling:
7 mL
NOTE
HOBt is optional, but is recommended for sequences susceptible to aspartimide formation.
WARNING
The Discover Bio reaction vessels should not be operated above 75
º
C.