Discover Bio
™
Manual Microwave Peptide Synthesizer
600423 • Revision 2 • August 2017
12
5.
When the desired volume of solvent has been added to the vessel, set the left knob to “N
2
Purge
(Wash)” to purge any solvent remaining in the lines. When all solvent has been purged into the
vessel, set the left knob to “Stop”.
6.
Allow the resin to swell for approximately 15 minutes. Optionally, the resin can be bubbled during
swelling by setting the right knob to “N
2
Bubbling” after the solvent has been added.
7.
After the resin has swollen, drain the solvent from the reaction vessel by setting the left knob to
“N
2
Purge (Wash)” the right knob to “Waste”. Once all the solvent has drained from the vessel, set
the right knob to “Stop”.
Performing a Deprotection
1. Set the left knob to “Deprotect” to begin adding deprotection solution to the reaction vessel.
2.
When the desired volume of deprotection solution has been added to the vessel, set the left knob
to “N2 Purge (Deprotect)” to purge any remaining deprotection solution in the lines. When all
reagent has been purged into the vessel, set the left knob to “Stop”.
3. Set the right knob to “N2 Bubbling”.
4.
Ensure the fiber-optic probe is fully inserted into the thermowell.
5.
Insert the reaction vessel into the Discover cavity. Load the Deprotection microwave method, and
press the
Play/Pause key to begin the method.
6.
When the microwave method is finished, remove the reaction vessel from the Discover cavity and
place it in the vessel holder. Set the left knob to “N2 Purge (Wash)” the right knob to “Waste”.
7.
When all the solvent has drained, set the right knob to “Stop”. Set the left knob to “Wash Solvent”
and add approximately 7 mL of solvent to the vessel, then set the left knob to “Stop”. Optionally,
to bubble the resin for a more efficient wash, turn the right knob to “N2 Bubbling” and allow the
resin to bubble for five seconds.
8.
Set the left knob to “N2 Purge (Wash)”, then set the right knob to “Waste” to drain the reaction
vessel. Set both knobs to “Stop”.
9. Repeat steps 7 and 8 two more times.
Performing a Coupling
1. Set the left knob to “Add” and the right knob to “Stop”.
2.
Dissolve the appropriate amino acid, activator, and activator base in a total volume of
approximately 4 mL.
NOTE
Purging the line will add an additional 1-2 mL of solvent to the reaction vessel.
NOTE
Purging the line will add an additional 1-2 mL of deprotection solution to the reaction vessel.
WARNING
The Discover Bio reaction vessels should not be operated above 75
º
C.