Büchi Labortechnik AG
Operation | 7
Operation Manual Sepiatec SFC-250
53/62
u
Define the number of fractions to be collected
and the fractionation time-scale.
u
Check the system settings in the system
window.
See Software, for more detailed information
about setting up methods, adjusting fractionation
conditions and specifying system settings.
7.5
Starting a run
NOTE
Depending on the selected parameters, an equilibration time of several minutes is
required to adjust to the set temperature profile. The shortest recommended time
period is 5 minutes. If a stable temperature profile has not yet been established
during this time, the equilibration time is automatically extended until either a
stable temperature profile has been established or a maximum duration of 15
minutes has been reached. If a stable temperature profile has not been established
after 15 minutes, the run is automatically stopped.
Navigation path:
➔
PARAMETER
Precondition:
R
Make sure there is enough CO
2
and solvents to complete the separation run of
the sample. The eluent consumption is highly dependent on the used method. To
prevent damage to the pumps, it is necessary to avoid running them dry.
R
Make sure the volume of the fraction bottles and the waste container are large
enough for the whole separation to avoid overfilling.
R
Make sure the collection bottle is empty and the collection bottle cap is tightly
screwed.
R
Make sure instrument is ready for a run.
R
Make sure the correct method and fraction conditions are selected.
u
Select the correct column, gradient and detection wavelength.
u
Tap the
[Pump Start]
button.
ð
The pumps are starting with the starting conditions of the selected gradient.
u
Tap the
[Injection]
button.
ð
The injection can be started when the set temperature conditions are reached.
7.6
Finishing a run
NOTE
After a run is finished, the pumps stop automatically and the CO
2
stop valve is
closed automatically. The temperature controllers and the back pressure regulator
will remain active.
