10 | Appendix
BÜCHI Labortechnik AG
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Operation Manual Pure Chromatography Instrument C-805
u
Adjust the flow rate according to your needs.
u
Select the radio button for Speed or Purity.
u
Tap the button
[Calculate]
.
ð
The display shows the results.
u
Tap the button
[Accept]
.
ð
The gradient is saved for a run.
ð
The dialog box closes.
10.3.4
LC-Transfer
The LC-Transfer tool converts an HPLC gradient into a Flash Chromatography gradi-
ent.
Specification of the sample needed:
One run on a HPLC column (in gradient elution mode)
Times %B changes (t1, t2 and t3)
Precondition:
R
The display shows the dialog
Navigator
. See Chapter 10.3.1 "Open the naviga-
u
Select the LC-Transfer tab.
u
Select the HPLC column type used from the drop-down list.
u
Enter the flow rate used in the HPLC separation.
u
Enter the following HPLC gradient conditions:
lower and higher %B
times t1, t2 and t3
u
Select the cartridge you wish to use for the Flash separation from the drop-down
list.
ð
The cartridge is selected.
ð
The display shows the default flow rate.
