10 | Appendix
BÜCHI Labortechnik AG
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Operation Manual Pure Chromatography Instrument C-805
10.3.2
TLC Silica
The TLC-Silica tool uses TLC separation data to provide a recommended gradient for
silica separations of 2 or 3 components.
Specification of the samples needed:
Two different solvent concentrations
Two separations
Silica TLC plates
Precondition:
R
The display shows the dialog
Navigator
. See Chapter 10.3.1 "Open the naviga-
u
Select the
TLC- Silica
tab.
u
Select the radio button for two or three components.
u
Select the weaker solvent at drop-down list A.
u
Select the stronger solvent at drop-down list B.
u
Enter the solvent B concentrations used in the TLC separations.
u
Measure the distance the spot moved on the silica TLC plates.
u
Divide the measured value by the distance the solvent traveled.
ð
This is your Rf- value.
u
Enter the value in the Rf entry field.
u
Select the column you wish to use from the drop-down list
ð
The column is selected.
ð
The display shows the default flow rate.
u
Adjust the flow rate according to your needs.
u
Select the radio button for Speed or Purity.
u
Tap the button
[Calculate]
.
ð
The display shows the results.
