Branson Sonifier 250, Руководство пользователя, Страница: 29 / 135

4-4 100-214-239 Rev. A
Installation and Setup
CAUTION
j
DO NOT allow the horn or microtip to contact lab stands, beakers, test tubes
or similar objects. Microtip failure may result. Breakage of glassware may
result in the loss of a specimen.
Disruptor horns are the base for many microtip applications. Disrupter horns are threaded so
that they may also be inserted into a Flow-Thru chamber attachment (see page 4-6). When
threaded together, a closed chamber is formed between the Tissue Disruptor and the cavity of
the Flow-Thru Attachment. The Tissue Disruptor may also be used alone.
The Tapered Microtip attaches directly to a standard 1/2 inch tapped Disruptor horn. The
amplitude at the end of a tapered tip is three and one-half times greater than that of the stan-
dard horn. The tapered tip is recommended for difficult applications such as spores, fungi,
yeast, muscle, and connective tissue. Excellent results can be achieved on volumes ranging
from 3 to 20 ml in a comparatively short period of time. The diameters of the tapered micro
tips are 1/8 inch (3.2 mm), 3/16 inch (4.8 mm), and 1/4 inch (6.4 mm).
The stepped microtip is a two-piece unit, consisting of a Coupling Section and a lower double-
step tip. Because the coupling section is attached directly to the converter, the standard disrup-
tor horn must be removed prior to using the stepped microtip. Recommended for use on
extremely small volumes, the stepped microtip can be used to treat volumes ranging from 0.5
to 20 ml. Applications for this tip include red and white blood cells, tissue culture cells, HeLa
cells, and the complete range of cells that have low to medium resistance to breakage.
To prevent foaming or aerosoling while processing small quantities with the tapered or stepped
microtips, the use of a conical-shaped tube such as a reaction vial or a cut down centrifuge tube
is recommended.
CAUTION
j
When using microtips, do not exceed a maximum Amplitude Control setting
of 70%. The microtip will break if driven at higher amplitudes.
Tissue Disruptor
Designed for disintegration of difficult tissues, this stainless steel accessory has a specially con-
structed cell bottom that holds up to six grams of tissue. A water jacket may be provided for
cooling.