Integrated Multi-parameter Sensor Operation manual
44
obtained by
water and seawater standard test methods
; B, 0.3 mg/L Rhodamine B stain solution.
Remove the probe from the distilled water and wipe it into a calibration container containing 0.3 mg/L of
Rhodamine B stain solution or water sample. The probe should be at least 2 cm below the solution, and
the probe mirror should not have air bubbles.
When the AD value in the software interface is stable, enter the chlorophyll value of 0.3 mg/L Rhodamine
B stain solution at the corresponding temperature in the “Calibration” box (0.3 mg/L rhodamine B as a
function of temperature) Table, as shown in Table 1) or experimentally measured chlorophyll values, then
click on "Solution Calibration".
3.
After calibration is complete, rinse the sensor and calibration container with water and dry.
You can also directly enter the value in the “Correction Parameters” box and click “factor” and
“deviation” to calibrate the factor and deviation value.
Important note: Shading should be used during the calibration process; the temperature of the
standard solution should be determined each time the counter standard is measured to determine the
chlorophyll value of the standard solution.
Table 1 Algae chlorophyll as a function of temperature for 0.3 mg/L rhodamine B
Temperature
(
℃
)
Chlorophyll
（
μg/L
）
Temperature
(
℃
)
Chlorophyll
（
μg/L
）
30
43.51
18
65.06
28
47.23
16
67.82
26
51.23
14
70.5
24
56.11
12
73.1
22
59.74
10
75.8
20
63.46
8
77.3
Note:
As shown in the table, 0.3mg/L Rhodamine B corresponds to 56.11μg/L chlorophyll at 24°C.
6.1.8.3 Blue Green Algae Sensor
From the Calibration menu, select the Blue Green Algae option to enter the Blue Green Algae Calibration
Program, as shown in the image below, and then ready to begin calibration.