4. Operation
9
the protocol chip is placed into the port, the
MiniTurbo
will recognize it automatically.
Figure 6. Protocol Chip.
4.4 Heated Lid
The heated lid heats the air in the upper part of the sample vessels to a temperature higher than the
reaction mixture. This prevents condensation of the evaporated water vapor on the vessel walls
and keeps the concentration of the reaction mixture unchanged during the heating and cooling
cycles. The heater platen also applies pressure to the caps or sealing film on the vessels to prevent
vapor loss and cross contamination between samples.
4.5 Loading the Reaction Vessel
For optimal performance of the
MiniTurbo
, the recommended sample volume for 0.2 ml tubes is
20-50
μl.
Please make sure the tube caps are sealed tightly to prevent evaporation and overflow of
liquid. Make sure the tubes are pushed straight down into the well so that they are all at the same
level when the lid is closed.
Figure 7. Loading the reaction vessels.