98
| Chapter
6
:
Instrument Qualification Procedures
Fluorescence Plate Tests
BioTek Fluorescence Test Plate
on page 73 introduces the test plate and references its
user guide for the test methods. Use of the test plate is offered as an alternative to
conducting the fluorescence liquid tests described in the next section.
Requirements
Refer to the
Getting Started
section of the
Fluorescence Test Plate User Guide
for
information on the required materials and prerequisite tasks.
Test Procedure
The
Qualification Tests
section of the
Fluorescence Test Plate User Guide
contains a
procedure for cleaning the plate and then creating and running experiments based on
supplied Gen5 protocols.
As described in the user guide, when each experiment is finished, Gen5 exports the
measurement data to a prepared Microsoft Excel .xls file. The worksheet(s) within that file
calculate results and determine pass or fail. Identify the reader-specific Gen5 protocols on
the USB flash drive that came with the test plate. Use only those protocols that apply to
your reader model and your organization's qualification requirements.
Fluorescence Liquid Tests
Fluorescence Liquid Tests
, starting on page 74, describes the test methods, lists the
Gen5 protocol parameters, explains how to analyze the test results, and provides
troubleshooting tips in the event of test failure.
The tests presented in this section require specific microplates, solutions, and filter
cubes. Your laboratory may require a deviation from some of these tests. For example,
you may wish to use a different fluorescing solution or microplate.
If deviation from the tests as presented in this section is required, the following steps
should be taken the first time each test is run:
• Perform the tests exactly as described on the following pages.
• Rerun the tests using your particular plates, solutions, and so on. If results are
comparable, then the results from these tests will be your baseline for future tests.
• Document your new test procedure(s), and save all test results.
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.
Содержание Synergy LX
Страница 45: ...Repackaging and Shipping Instructions 21 Synergy LX User Manual 1501000N Revision A BioTek Instruments Inc ...
Страница 46: ...22 Chapter 2 Installation Synergy LX User Manual 1501000N Revision A BioTek Instruments Inc ...
Страница 55: ...Filter Cubes 31 Synergy LX User Manual 1501000N Revision A BioTek Instruments Inc ...
Страница 138: ...114 Appendix B Sample Reports Sample System Report Synergy LX User Manual 1501000N Revision A BioTek Instruments Inc ...
Страница 139: ...Sample System Report 115 Synergy LX User Manual 1501000N Revision A BioTek Instruments Inc ...