Biotek Synergy H1, Руководство оператора

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Decontaminate the Fluid Lines
1. Place a beaker with 20 mL of 0.5% sodium hypochlorite solution or 70% isopropyl
alcohol near SYRINGE 1 on the dispense module.
2. Place the SYRINGE 1 inlet tube in the beaker.
3. If you have not already done so, detach the dispense module’s outlet tubes from
the instrument. Place the ends of the outlet tubes in an empty beaker and set the
beaker next to the dispense module.
4. Launch Gen5, select System > Instrument Control , and click the Prime tab.
5. Select Dispenser 1 , enter a Volume of 5000 µL, and keep the default dispense
Rate.
6. Place the priming plate on the carrier.
7. Run two prime cycles, for a total of 10,000 µL.
8. Wait at least 20 minutes to allow the solution to disinfect the tubing.
9. Remove the inlet tube from the beaker of disinfectant solution.
10. From the Reader Control dialog, change the Volume to 1000 µL.
11. Run one prime cycle, to flush the disinfectant out of the fluid lines.
12. Empty the beaker containing the outlet tubes. Put the tubes back in the empty
beaker.
13. If sodium hypochlorite (bleach) was used, perform the next procedure, Rinse the
Fluid Lines .
Otherwise (or after performing the Rinse procedure), repeat steps 1–13 for
SYRINGE 2/Dispenser 2.
Rinse the Fluid Lines
Perform this procedure only if decontamination was performed using sodium
hypochlorite.
1. Place a beaker containing at least 30 mL of deionized water on the dispense
module.
2. Place the SYRINGE 1 or 2 inlet tube in the beaker.
3. If you have not already done so, place the outlet tubes in an empty beaker.
4. From the Reader Control dialog, select Dispenser 1 or 2, set the Volume to 5000
µL, and keep the default dispense Rate.
5. Run five prime cycles, for a total of 25,000 µL.
6. Pause for 10 minutes and then run one prime cycle with 5000 µL. This delay will
allow any residual sodium hypochlorite to diffuse into the solution and be flushed
out with the next prime.
7. Empty the beaker containing the outlet tubes.
8. Wipe all surfaces with deionized water.
Synergy H1 Operator's Manual
Decontamination | 69