Biotek Synergy H1, Руководство оператора

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If an Absorbance Test Plate is not available, Absorbance Liquid Test 2 may be
conducted to test the instrument's alignment, repeatability, and accuracy by preparing a
series of solutions of varying OD values as described on page
116 .
Absorbance Liquid Test 3 is an optional test offered for those sites that must have
proof of linearity at 340 nm. (Alternatively, the BioTek 340 nm Absorbance Test Plate
may be used; see page
75 .) This test is optional since the Synergy H1 has good “front-
end” linearity throughout the specified wavelength range. While the absolute values of
the OD cannot be determined by this test, the results will indicate if there is adequate
repeatable absorbance and a linear slope. This method is dependent upon proper dye
dilution and a skilled pipetting technique. It is expected that the first dilution (mid-level
solution) will have an absorbance value near 75% of that of the stock (high-level)
solution, and that the second dilution (low-level solution) will have an absorbance value
near 50% of that of the stock solution.
Gen5 Protocol Parameters
The information in this section represents the recommended reading parameters for the
referenced Gen5 protocol(s). It is possible that your tests will require modifications to
some of these parameters, such as the Plate Type.
i The Plate Type setting in each Gen5 protocol should match the actual plate in use.
Synergy H1 Abs Test 1.prt
Parameter Setting
Plate Type 96 WELL PLATE
Shake Step Linear, 4 minutes, default frequency
Two Read Steps
Kinetic loop (one per Read step) Set a Run Time/Interval combination to read the
plate five times with minimal delay
Detection Method Absorbance
Read Type Endpoint
Optics Type Monochromators
Read wells First Read step: A1..H2
Second Read step: A11..H12
Wavelength 405 nm
Read Speed Normal
Delay after plate movement 100 msec
Plate Out,In step between loops Text "rotate the plate 180 degrees"
78 | Chapter 7: Instrument Qualification Process
BioTek Instruments, Inc.