411829-L, Operation
April 2012
Page 4-15
4
4.5 Prepare and Run a Purification
4.5.1 Prepare the System for a Run
1. If no equilibration is to be performed and an internal dry loading technique is to be used (see
page 1-4), load the sample. See Table 4-3 on page 4-21 for cartridge loading capacity.
2. Load the cartridge (see Figure 4-10 on page 4-16) and collection rack(s) that you want to use.
3. If you want to use the sample loading pump (only available on Isolera LS), see “Prepare the
Sample Loading Pump for a Run (Only Isolera LS)” on page 4-17.
4. Ensure that a sufficient quantity of the correct solvent is present in each solvent reservoir and
that the waste reservoir has sufficient capacity for the run. To assign solvents and set reservoir
capacities and current levels, see page 4-32.
5. If you need to prime the system due to change of solvent or air bubbles, see page 4-33. Note
that all solvent inlets must be primed with solvent.
6. To turn on the detector lamp(s) in advance, press
Turn Lamp On
in the right-hand panel (in
the software).
NOTE
The UV lamp should be turned on approximately 7.5 minutes before
operation so it can warm up and stabilize.
If automatic rack allocation is enabled, the collection area must at all
times be loaded with racks in all positions.
All solvent inlets must be primed with solvent to achieve the specified
pump performance.
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