Biotage Isolera Four, Руководство пользователя

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411829-L, System Settings April 2012 Page 3-9
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• Automatic Vessel: The system allocates vessels in sequence, starting with the first vessel
in rack A, the second in rack A, and so forth. With this rack allocation method, racks can be
shared by several runs but the collection tray cannot be shared by several users.
When enabling automatic rack allocation on an Isolera Four system, the following rules also apply:
• All racks on the collection tray must be of the same type. The rack type to be used is
decided by the first purification that is queued up.
• Allocation only when needed. No rack or vessel allocation is performed when a
purification is queued up. When the run is started racks or vessels are allocated one at a time
as they are needed.
• Always replace all racks when asked to. If the system runs out of vessels, you will be
asked to replace all racks in the collection area at the same time (even if some of the racks
have been used by previous runs).
To enable or disable automatic rack allocation:
1. Select the Runtime tab in the right-hand panel.
2. Select the Rack Allocation Mode text box.
3. To enable automatic rack allocation, select Automatic or Automatic Vessel .
4. To disable automatic rack allocation, select Manual .
3.6.3 Enable or Disable Peak Mode
If Peak Mode is enabled, all fractions of a peak will have the same color in the status view (the
Status tab in the Chemistry mode).
1. Select the Runtime tab in the right-hand panel.
2. To enable or disable Peak Mode in the status view, select the Peak Mode text box and select
Yes or No .
3.6.4 Enable or Disable Auto Extend
If Auto Extend is enabled and the system is collecting fractions and remaining light absorption is
detected when the system nears the end of a purification, the system enters the Auto-Extend
mode. This extends the gradient purification stage of the run with 25% of the total gradient length
using the final conditions in the method.
1. Select the Runtime tab in the right-hand panel.
2. To enable or disable Auto Extend, select the Auto Extend text box and select Yes or No .
3.6.5 Enable or Disable Monitoring of Reservoirs
If the Solvent Monitoring and Waste Monitoring options are enabled, the system will issue a warning
when it is time to replenish a solvent and empty a waste reservoir.
To enable solvent and/or waste monitoring:
1. Select the Runtime tab in the right-hand panel.
2. To track solvent levels, select the Solvent Monitoring text box and select the level when the
processing shall be paused and refill requested.
3. To track waste levels, select the Waste Monitoring text box and select the level when the
processing shall be paused and emptying requested.
4. If using an Isolera Four system, ensure to select the number of waste reservoirs to be used in
the Waste Reservoir text box. The waste can either be pooled into a single waste reservoir
(insert all four waste outlet tubes into a single waste reservoir) or collected in four waste
reservoirs (insert each waste outlet tube into a single waste reservoir).
5. If you want an audible alarm to sound when it is time to replenish a solvent or empty a waste
reservoir, check that the Audible Alarm option is enabled (see page 3-8).