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PERIFUSION SYSTEM

MODEL No: PERI-4.2

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USER MANUAL

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Содержание PERI-4.2

Страница 1: ...1 PERIFUSION SYSTEM MODEL No PERI 4 2 ______________________________________________________ USER MANUAL ______________________________________________________...

Страница 2: ...YSTEM OVERVIEW 11 5 1 BASIC PERIFUSION SCHEMATIC 11 5 2 VALVE MANIFOLD 12 5 3 PERISTALTIC PUMP 14 5 4 PERIFUSION CHAMBER 14 5 5 COLLECTION TRAY 15 6 PERIFUSION SYSTEM BASICS 16 6 1 POWER UP 16 6 2 LOG...

Страница 3: ...ture that accompanies the device This symbol warns of moving parts that may cause personal injury This symbol is used when two people are needed to do the task 1 2 Machine Information In the spaces pr...

Страница 4: ...ce with the manufacturer s instructions See Site Preparation in section 1 5 for details 3 Do not defeat the safety purpose of the polarized or grounding type plug A polarized plug has two blades with...

Страница 5: ...t The following items are included with the purchase of a Perifusion system Qty Reference Description 1 86610610 Power Cord 12 PERI CASSETTE Pump cassettes 12 PERI CHAMBER Perifusion Chambers 1 PERI T...

Страница 6: ...uring the stimulation of pancreatic islets The machine accurately controls temperature flow stimulus source and sample collection in up to 8 channels in parallel The Perifusion system is designed to e...

Страница 7: ...7 3 Machine parts identification Figure 3 Perifusion system side view Figure 4 Perifusion system front view...

Страница 8: ...tic pump Main pump 4 Temperature control inlet outlets x6 5 Perifusion chamber 6 Sample collection tray 7 Alarm buzzer 8 Tray cooling diaphragm pump 9 Power cable 10 Incubator cover 11 12 Touch Screen...

Страница 9: ...ovides the precise resolution needed in perifusion experiments A set of each type is included with this machine Item Description Reference A Silicone Tubing 0 040in ID 0 085in OD 89068 462 B Two Stop...

Страница 10: ...oplets 4 L ensure optimal resolution of the dynamic insulin response Figure 8 Perifusion Nozzles 4 5 Collection well plates The collection well plate is where the cell secretions and the perfusate are...

Страница 11: ...mperature controlled environment 2 VALVE MANIFOLD An automated valve manifold handles the source selection for each channel The manifold has 8 input channels 1 2 3 8 and four output channels A B C and...

Страница 12: ...Minimizing this problem was a priority at the time of developing the new Perifusion system The result was a self contained 32 valves 8 in to 4 out manifold The manifold can change the source for every...

Страница 13: ...ected source 0 represents a closed channel Example The manifold state A2 B3 C8 D0 means output A will be from source 2 output B will be from source 3 output C from source 8 and the outputs of channel...

Страница 14: ...scription of tubing setup can be found in section 7 5 5 4 Perifusion chamber Sources delivered by the pump will react with the cells inside the perifusion chamber The cells are contained inside the ch...

Страница 15: ...itioning mechanism 3 In addition the collection plate can be refrigerated by circulating fluid through the available ports 4 The Perifusion system features a new deep collection tray that allows the u...

Страница 16: ...s entirety This machine has been designed with alarm features that will help the user identify and correct potential problems Alarm messages will be displayed at the bottom of the screen A detailed de...

Страница 17: ...reate a new user account press the Add User Button When the Add User button is pressed the New User Account fields on the right of the screen will be enabled Upon clicking on any field a pop up keyboa...

Страница 18: ...st Page In this page you will also see the User account the Software Version installed in your perfusion machine and the machine Status Bar at the bottom The Status Bar will display any alarms or impo...

Страница 19: ...ht This icon should change when the tray is moved between Landscape and Portrait orientations MOTION The motion controls manipulate the position of the tray by enabling direct access to the motors Sim...

Страница 20: ...valuable sub system for automating a Perifusion experiment It is composed by the precision peristaltic pump and the 8 to 4 manifold The pump can be tested by assigning a value to the Pump Flow rate b...

Страница 21: ...structions on operating Orbitor enabled machines TIMING The timing controls are used for special protocol execution sequences ACTION REQUIRED ALARM If the user does not want or can t use the automatic...

Страница 22: ...confirming your choice and reminding you the positions of the chambers in the chamber rack inside the incubator As a general rule chambers should be placed on the rack starting from the right most po...

Страница 23: ...een you will find a of Plates This number will tell you the number of well plates required to complete the protocol This will help you have all the plates in hand to avoid unnecessary delays Creating...

Страница 24: ...to the inlet tube of the top cap 9 Repeat for all chambers being used You should end up with two halves as shown in Figure 2 of the screen Figure 29 Perifusion Chamber Assembly Once you have both hal...

Страница 25: ...ve protocol You will be able to monitor the valve sequence followed by the machine as it performs the priming The progress will be displayed on the bottom bar Figure 31 Priming page 7 7 Cell loading W...

Страница 26: ...ontrol Once the system has been prepared the incubator must be heated up to the desired temperature and the collection tray must be cooled down to preserve the analytes Follow the on screen instructio...

Страница 27: ...en you reach this screen the cells should be in the chambers the manifold and should be primed with the input solutions and the temperature of the incubator and tray should be at their set points On t...

Страница 28: ...low you to load a 96 well plate into the sample tray Press the PLAY button once more to begin the protocol Figure 37 Execution Controls ALARMS AND INDICATORS This section of the protocol monitor displ...

Страница 29: ...he timing and progression of the experiment Since the experiment has many chronological levels these indicators will help you identify where you are in your protocol The first level locates you in the...

Страница 30: ...30 AUC Area Under Curve...

Страница 31: ...the chamber from the micro centrifuge tube 6 Close the lid of the micro centrifuge tube 7 Centrifuge at 300g for 5 minutes 8 Aspirate the supernatant leaving beads and islets behind At this point the...

Страница 32: ...mixture of 9 parts distilled water and 1 part bleach based disinfectant 3 Rinse once more with de ionized water 4 Dry by drawing air remove input tubing from the manifold Figure 43 Cleaning page Durin...

Страница 33: ...sed The plate maps are in tabular form representing the 96 well plate 8 x 12 and each cell represents an individual well Each cell contains two pieces of information The color indicates which solution...

Страница 34: ...ng the above solutions the pH 7 08 8 Bring volume to 200ml with ddH2O 9 Add 0 4g 0 2 of BSA Sigma Cat A7888 OR A6003 10 Filter Sterilize through 0 22 filter Notes a When making Solution 2 dissolve CaC...

Страница 35: ...kept refrigerated for up to 4 weeks Bead Suspension Use Follow these instructions before using the bead suspension in a perifusion chamber preparation Warm bead suspension to 37 using an incubator or...

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