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Technical Specifications

SPECIFICATION

VALUE

Sample Capacity

9 Wells

Reaction Volume per Well

20µL

Total Channels

3

Franklin™ one9 Fluorophore*

FAM / SYBR (Green)

Franklin™ two9 Fluorophores*

FAM / SYBR (Green), ATTO647N (Red)

Franklin™ three9 Fluorophores

FAM / SYBR (Green), TexasRedX (Amber),

ATTO647N (Red)

System Control & Data Transfer

Wireless (BLE)

Integrated Barcode Scanner

Yes

Max Samples per Run

9

Max PCR Targets per Run

27

Weight

1.20 kg / 2.65 lb

Franklin™ PCR Thermocycler User Manual

© Biomeme, Inc. 2019

Version 1.2

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Содержание Franklin

Страница 1: ...Franklin Real Time PCR Thermocycler Last Updated 02 24 2020 ...

Страница 2: ...ng Battery Status 8 Enabling Disabling Bluetooth BLE 9 Recovering Reattaching Test Data 10 Transferring Data 14 Loading Sample into Go Strips 15 Placing into Franklin Thermocycler 16 Maintenance Cleaning 17 Safety Notice 17 General Safety Warnings 18 Declaration of Conformity 20 Troubleshooting 21 Disclaimer 2 4 Franklin PCR Thermocycler User Manual Biomeme Inc 2019 Version 1 2 ...

Страница 3: ...tiplex real time detection of up to 27 targets from 1 sample or test 9 samples for up to 3 targets each Just under 3 lbs hand held and battery operated for maximum portability enabling a full day s work out in the field on a single charge Franklin comes in 3 different variations depending on the color channel detection you require see Technical Specifications for details Franklin PCR Thermocycler ...

Страница 4: ...SYBR Green Franklin two9 Fluorophores FAM SYBR Green ATTO647N Red Franklin three9 Fluorophores FAM SYBR Green TexasRedX Amber ATTO647N Red System Control Data Transfer Wireless BLE Integrated Barcode Scanner Yes Max Samples per Run 9 Max PCR Targets per Run 27 Weight 1 20 kg 2 65 lb Franklin PCR Thermocycler User Manual Biomeme Inc 2019 Version 1 2 ...

Страница 5: ...Outdoor Indoor or Outdoor in a Covered Area Pollution Degree 2 Degree of Ingress Protection Keep 5 cm Clearance Around the Thermocycler for Proper Performance Note The Franklin one9 is limited to the FAM SYBR Green channel and the two9 is limited to the FAM SYBR Green and ATTO647N Red channels only You can upgrade your thermocycler s at any time without returning your device Franklin PCR Thermocyc...

Страница 6: ...6 Thermocycler Button Layout There are a total of 4 buttons located on the top of your Franklin thermocylcer Franklin PCR Thermocycler User Manual Biomeme Inc 2019 Version 1 2 ...

Страница 7: ...ates remaining battery is between 81 and 100 1 blinking indicates charging 1 solid indicates run start to 9 complete 2 solid indicates run is between 10 and 31 complete 3 solid indicates run is between 32 and 53 complete 4 solid indicates run is between 54 and 75 complete 5 solid indicates run is between 76 and 99 complete 1 solid indicates remaining battery is between 0 and 20 5 blinking indicate...

Страница 8: ...ng Battery Status Note To preserve your smartphone s battery life disconnect from the thermocycler when it s not in use If your battery is running low simply plug the AC power adapter into an outlet and insert the power connector into the back of your thermocycler The LED on the back of the thermocycler will illuminate blue If you re unable to turn your thermocycler off using the power button on t...

Страница 9: ...ttery at least once every six months The thermocycler should not be left without charging for extended periods of time If your device has not been charged in more than six months and you cannot get the thermocycler to turn back on please contact support biomeme com Enabling Disabling Bluetooth BLE Bluetooth can be turned on or off at any time by pressing and holding the Bluetooth button on the top...

Страница 10: ... re wondering how to retrieve your test results Below are some common scenarios and the steps to take to get your results You can find this same protocols with their connected How To videos on our Help Desk https help biomeme com reattach recover guide Scenario Reference Table Biomeme Go App Open Closed Franklin Thermocycler On A B Off C D Note DO NOT push Stop Run in the app This will lose any ru...

Страница 11: ...rn off the thermocycler and close the Biomeme Go app 2 Follow the steps described in Scenario D below If you re still having problems recovering and or reattaching your test data please contact support biomeme com My Biomeme Go app is returning a WRONG THERMOCYCLER error 1 Turn off the thermocycler and close the Biomeme Go app 2 Follow the steps described in Scenario D below A Thermocycler ON App ...

Страница 12: ...ycler ON App CLOSED 1 Relaunch the Biomeme Go app by selecting the icon on your smartphone s home screen 2 Press the Bluetooth button on top of the thermocycler to turn OFF the Bluetooth connection 3 Press the Bluetooth button on the top of the thermocycler to turn the Bluetooth connection back ON 4 From the app s home screen tap Incomplete Runs 5 Select your test from the list of incomplete runs ...

Страница 13: ...eme Go app 6 Select your thermocycler 7 Please wait while your run data is transferred Note This assumes your test was completed before the thermocycler powered off It is usually not possible to reattach and recover the run data if the thermocycler shut off during the run Please always ensure your device has at least 20 battery power before starting your run D Thermocycler OFF App CLOSED 1 Press t...

Страница 14: ...from the list of incomplete runs 8 Press the Scan button in the app and select your thermocycler 9 Tap Connect via BLE 10 Tap the Reattach Test button 11 Please wait while your run data is transferred Note This assumes your test was completed before the thermocycler powered off It is usually not possible to reattach and recover the run data if the thermocycler shut off during the run Please always...

Страница 15: ...ake sure to only copy and paste Loading Sample into Go Strips Attention Contents of the Go Strip may shift during transport When starting to work with your test make sure the cake of the lyophilized reagent rests at the bottom of the Go Strip wells Tap the bottom of the sealed Go Strip gently but firmly against a solid surface before removing the foil seal and adding your sample Tear open the foil...

Страница 16: ... the lid of the thermocycler is closed it will secure the caps into place sealing the PCR reaction It s important to make sure your Go Strip is oriented correctly when placing it into your thermocycler Make sure the strip connections that are visible through the void filling cap cutouts are facing the back of your thermocycler as shown in the illustration below then close the lid Franklin PCR Ther...

Страница 17: ...your Go Strips and void filling caps to avoid liquid splatter Maintenance Cleaning The Biomeme Franklin thermocycler is maintenance free and has no serviceable parts In the case of thermocycler failure or damage please contact support biomeme com The outside of the Franklin thermocycler can be cleaned using 70 ethanol solution which must be sprayed on a cloth rather than directly on the Franklin t...

Страница 18: ...ty of the operator The instrument and its equipment should be operated maintained stored and as directed in this document Failure to comply may impair the protection provided by the instrument and its ancillary equipment General Safety Warnings Do not modify the instrument hardware The system is not user serviceable by the user in any circumstances Do not place the instrument near liquid filled co...

Страница 19: ...ng or removing cuvettes Electrical Safety Warnings Unplug AC power cord from the wall outlet in case of an emergency SYMBOL DESCRIPTION Caution possibility of electric shock Caution hot surface Caution Franklin PCR Thermocycler User Manual Biomeme Inc 2019 Version 1 2 ...

Страница 20: ... and ensure that it is recycled in a manner that protects human health and the environment For more information about where you can drop off your waste equipment for recycling please contact your local city office your household waste disposal service or the shop where you purchased the product Declaration of Conformity This declaration of conformity is issued for Product Biomeme Franklin qPCR The...

Страница 21: ...f the manufacturer Troubleshooting Why is my Bluetooth not connecting If you are having trouble connecting ensure that you enabled Bluetooth on both your smartphone and thermocycler Why is my thermocycler not showing up in the connection list Ensure that Bluetooth is turned on The LED next to the button should be illuminating blue If your thermocycler is still not showing try scanning multiple tim...

Страница 22: ...esulting in no Cq values baseline or graph of smooth data The raw data and information about your run is still exportable through the xlsx spreadsheet however My thermocycler turned off during a test run If your thermocycler turns off during a test then the thermocycler battery may be dead and the unit should be plugged into power Your connection to the smartphone will also be lost If this happens...

Страница 23: ... button Doing so will prompt the mobile app to ask you to confirm that you would like to stop the test in progress Upon stopping your run will be saved to the current point and available in the test results section of the mobile app The thermocycler failed to start test If your run fails to start the app will return to the home screen and have you restart the setup of your test Restart the thermoc...

Страница 24: ...de you with a unique 16 character unlock code per unit to be entered using the Biomeme mobile app Please note your smartphone must be connected to the thermocycler via Bluetooth or serial in order to complete the upgrade What should I do if I receive a heater error message Retry running your test but if the error persists please contact support biomeme com Disclaimer For Research Use Only Not for ...

Страница 25: ...may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This device contains FCC ID XPYNINAB1 IC 8595A NINAB1 Warning Changes or modifications not expressly approved by the party responsible for compliance could void the use...

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