CoolCell
®
SV2
Instructions for Use
BCS-172
CoolCell
®
SV2
Instructions for Use
BCS-172
Controlled-rate cell freezing container
Controlled-rate cell freezing container
Quick Start
• The 12 chambers and 2ml serum vials should be dry to avoid tube sticking upon freezing.
• Make sure the core (black ring) is at room temperature and seated in the bottom of the central cavity.
• Place sample vials containing 1ml of cell suspension in each well. For reproducible freezing profiles, each
well should contain a filled vial; “blank” vials containing 1 ml of freezing medium should be placed in
empty chambers if freezing less than 12 vials of cell suspension.
• Check that the tubes slide in and out freely.
• Fully seat the lid on CoolCell SV2 container.
• Place the CoolCell SV2 container into a -80
o
C
freezer or dry ice locker. Ensure that there is at least one
inch of free space clearance around the CoolCell SV2 module.
• Freeze for four hours before transferring samples to archive storage.
Transferring frozen samples to archive storage
• Prepare an insulated pan or container with a one inch (2.5cm) layer of pulverized or pellet dry ice.
• Remove CoolCell SV2 container from the freezer and gently remove the lid using a twisting and rocking
motion.
• Vial tops will be exposed once lid is removed and vials should be quickly extracted and placed onto the
dry ice.
Special Notes:
• Always use dry ice to transfer frozen vials containing cells to permanent storage to avoid temperature
rise and cell damage. Vial contents can rise from -80
o
C
to over -50
o
C
in less than one minute if exposed to
room temperature air.
• It is strongly recommended that all frozen cell cultures be checked for viability before the stock culture is
terminated.
Recycling CoolCell SV2 to room temperature
The CoolCell SV2 container is ready to freeze again as soon as the core (black ring) is at room temperature.
To rapidly recycle CoolCell SV2 container to room temperature, remove the center solid core ring by inverting
and tapping on a surface. CoolCell SV2 body and lid will return to room temperature in 10 to 15 minutes.
Check that all chambers are dry. Dry the core ring before re-inserting into the central chamber.
About CoolCell SV2
The CoolCell SV2 contianer, in combination with a -80
o
C
freezer or dry ice locker, will provide the freezing
rate of -1
0
C
per minute that is ideal for cryo-preservation of most cultured cell lines. CoolCell SV2 design
Quick Start
• The 12 chambers and 2ml serum vials should be dry to avoid tube sticking upon freezing.
• Make sure the core (black ring) is at room temperature and seated in the bottom of the central cavity.
• Place sample vials containing 1ml of cell suspension in each well. For reproducible freezing profiles, each
well should contain a filled vial; “blank” vials containing 1 ml of freezing medium should be placed in
empty chambers if freezing less than 12 vials of cell suspension.
• Check that the tubes slide in and out freely.
• Fully seat the lid on CoolCell SV2 container.
• Place the CoolCell SV2 container into a -80
o
C
freezer or dry ice locker. Ensure that there is at least one
inch of free space clearance around the CoolCell SV2 module.
• Freeze for four hours before transferring samples to archive storage.
Transferring frozen samples to archive storage
• Prepare an insulated pan or container with a one inch (2.5cm) layer of pulverized or pellet dry ice.
• Remove CoolCell SV2 container from the freezer and gently remove the lid using a twisting and rocking
motion.
• Vial tops will be exposed once lid is removed and vials should be quickly extracted and placed onto the
dry ice.
Special Notes:
• Always use dry ice to transfer frozen vials containing cells to permanent storage to avoid temperature
rise and cell damage. Vial contents can rise from -80
o
C
to over -50
o
C
in less than one minute if exposed to
room temperature air.
• It is strongly recommended that all frozen cell cultures be checked for viability before the stock culture is
terminated.
Recycling CoolCell SV2 to room temperature
The CoolCell SV2 container is ready to freeze again as soon as the core (black ring) is at room temperature.
To rapidly recycle CoolCell SV2 container to room temperature, remove the center solid core ring by inverting
and tapping on a surface. CoolCell SV2 body and lid will return to room temperature in 10 to 15 minutes.
Check that all chambers are dry. Dry the core ring before re-inserting into the central chamber.
About CoolCell SV2
The CoolCell SV2 contianer, in combination with a -80
o
C
freezer or dry ice locker, will provide the freezing
rate of -1
0
C
per minute that is ideal for cryo-preservation of most cultured cell lines. CoolCell SV2 design
