CoolCell
®
LX
Instructions for Use
CoolCell
®
LX
Instructions for Use
Controlled-rate cell freezing container for 1ml or 2ml cryovials
Controlled-rate cell freezing container for 1ml or 2ml cryovials
Quick Start
• The 12 chambers and cryovials should be dry to avoid tube sticking upon freezing.
• Make sure the core (black ring) is at room temperature and seated in the bottom of the central cavity.
• Place sample vials containing 1 ml of cell suspension in each well. Each well should contain a filled vial. If
freezing batch is fewer than 12 vials, fill each empty well with a BioCision CoolCell Filler Vial (2ml vial, part
number BCS-3105) or other vial that contains equivalent volume of freezing media.
Note: Cell suspensions can be inserted into a room temperature CoolCell and successfully preserved. For optimal
results, CoolCell should be at the same temperature as your cell suspensions.
• Check that the tubes slide in and out freely.
• Fully seat the lid on CoolCell LX
• Place CoolCell LX upright into a -80
O
C freezer or dry ice locker. Ensure that there is at least one inch of
free space clearance around CoolCell LX module.
• Freeze for four hours before transferring samples to archive storage.
Transferring frozen samples to archive storage
• Prepare an insulated pan or container with a one inch (2.5cm) layer of pulverized or pellet dry ice.
• Remove CoolCell LX from the freezer and gently remove the lid using a gentle twisting and rocking motion.
• Vial tops will be exposed once lid is removed and vials should be quickly extracted and placed onto the
dry ice.
Special Notes:
• Always use dry ice to transfer cryovials containing cells to permanent storage to avoid temperature rise
and cell damage. Cryovial contents can rise from -8o
O
C to over -50
O
C in less than one minute if exposed to
room temperature air.
• It is strongly recommended that all frozen cell cultures be checked for viability before the stock culture is
terminated.
Recycling CoolCell LX to room temperature
CoolCell LX is ready to freeze again as soon as the foam body and core (black ring) are at room temperature.
To rapidly recycle CoolCell LX to room temperature, remove the center solid core ring. CoolCell LX body and
lid will return to room temperature in 10 to 15 minutes. Check that all chambers are dry. Dry the core ring
before re-inserting into the central chamber.
Quick Start
• The 12 chambers and cryovials should be dry to avoid tube sticking upon freezing.
• Make sure the core (black ring) is at room temperature and seated in the bottom of the central cavity.
• Place sample vials containing 1 ml of cell suspension in each well. Each well should contain a filled vial. If
freezing batch is fewer than 12 vials, fill each empty well with a BioCision CoolCell Filler Vial (2ml vial, part
number BCS-3105) or other vial that contains equivalent volume of freezing media.
Note: Cell suspensions can be inserted into a room temperature CoolCell and successfully preserved. For optimal
results, CoolCell should be at the same temperature as your cell suspensions.
• Check that the tubes slide in and out freely.
• Fully seat the lid on CoolCell LX
• Place CoolCell LX upright into a -80
O
C freezer or dry ice locker. Ensure that there is at least one inch of
free space clearance around CoolCell LX module.
• Freeze for four hours before transferring samples to archive storage.
Transferring frozen samples to archive storage
• Prepare an insulated pan or container with a one inch (2.5cm) layer of pulverized or pellet dry ice.
• Remove CoolCell LX from the freezer and gently remove the lid using a gentle twisting and rocking motion.
• Vial tops will be exposed once lid is removed and vials should be quickly extracted and placed onto the
dry ice.
Special Notes:
• Always use dry ice to transfer cryovials containing cells to permanent storage to avoid temperature rise
and cell damage. Cryovial contents can rise from -8o
O
C to over -50
O
C in less than one minute if exposed to
room temperature air.
• It is strongly recommended that all frozen cell cultures be checked for viability before the stock culture is
terminated.
Recycling CoolCell LX to room temperature
CoolCell LX is ready to freeze again as soon as the foam body and core (black ring) are at room temperature.
To rapidly recycle CoolCell LX to room temperature, remove the center solid core ring. CoolCell LX body and
lid will return to room temperature in 10 to 15 minutes. Check that all chambers are dry. Dry the core ring
before re-inserting into the central chamber.
