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BioChain

Institute Inc.

Automated Nucleic Acid Preparation System

AnaPrep 12

Catalog No.: Z1321001

Rev. Date: Feb 12, 2016

Manual No. Z2001
Version: Z1321001UB (MV1.2)
For General Laboratory Use Only

Instruction Manual

Содержание AnaPrep 12

Страница 1: ...Chain Institute Inc Automated Nucleic Acid Preparation System AnaPrep 12 Catalog No Z1321001 Rev Date Feb 12 2016 Manual No Z2001 Version Z1321001UB MV1 2 For General Laboratory Use Only Instruction Manual ...

Страница 2: ...System 8 1 2 Contents of Reagent Kits 9 1 3 Operating Environment Condition 10 1 4 Unpack the AnaPrep 12 System 13 1 5 Overall View 15 2 0 GETTING STARTED 17 2 1 Turning on the power 18 2 2 Preparation 18 2 3 Extraction 19 2 4 After Extraction Operation 27 3 0 Cleaning Maintaining the AnaPrep 12 Instrument 28 4 0 Troubleshooting 29 5 0 Specifications 32 APPENDIX 33 ...

Страница 3: ...g maintenance requirement This guide contains important information regarding the safe use of the AnaPrep 12 system Please read this manual carefully especially the Safety Information before operating the system for the first time If you have questions about how to install or operate the system please contact our certified distributors agents or email to our technical support center techsupport bi...

Страница 4: ...rial CAUTION This symbol is used to indicate that non compliance procedures from specified instructions may lead to personal injury or may cause damage to the instrument Important Important indicates that the operator user should pay attention to the specified instructions or prohibited actions Note Note indicates additional useful information HOT SURFACE This symbol is used to label potentially h...

Страница 5: ...ou disconnect the plug from the outlet be sure to hold the power plug itself Do not pull the power cable For maintenance disconnect the power plug from the outlet Avoid contact with instrument or electrical cord and plug during storms Do not pour any liquid on the AnaPrep 12 system Do not place any objects containing liquid on the AnaPrep 12 system Doing so may cause a device failure fire or elect...

Страница 6: ...in Institute Inc replacement parts should be used If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired About Reagent Kits CAUTION When handling any of the kits refer to the respective handbooks Reagents in each kit should be handled by observing the safety information and precautions specific to the kit Extraction should be...

Страница 7: ...proper incineration sterilization and or disinfection When using a third party to dispose of hazardous waste be sure to outsource this work to an agency that is licensed to handle medical waste BioChain Service Center For technical issues or instrument maintenance please contact our service center BioChain Institute Inc 39600 Eureka Dr Newark CA 94560 USA 1 510 783 8588 1 510 783 5386 techsupport ...

Страница 8: ...1001UB Active Date 02012016 Page 8 1 Installation 1 1 Composition of the AnaPrep 12 system Check that the following are included in the package Contact your local representative if you notice any missing part s A B C D E ...

Страница 9: ... D Barcode Reader 1 E User s Manual 1 Please inspect all components as soon as you receive the system If any damage is found please contact your local representative for immediate support Note BioChain s global warranty does not cover damage from transportation or improper operation 1 2 Contents of Reagent Kits A B ...

Страница 10: ... Note Reagent kits are purchased separately Please contact your local representative for further information The contents of the reagent kits will be varied For more details please refer to the specific handbook enclosed with each reagent kit 1 3 Operating Environment Condition Use the AnaPrep 12 system in a location that meets the following conditions D C E F G ...

Страница 11: ...ry Surrounding area must be well ventilated and not dusty Temperature must be kept at a constant rate Warming a cold room suddenly or moving the AnaPrep 12 system from a room with low temperature to a warm room may cause condensation inside the device resulting in abnormal operation Temperature and humidity must be kept within the specified range far from water taps water heaters humidifiers air c...

Страница 12: ...ce such as a slanted surface or in a place subject to vibrations It may cause personal injury or device failure Do not use the AnaPrep 12 system in direct sunlight or close to a heating device It may shorten the life of the AnaPrep 12 system or cause equipment malfunction Operating Conditions Items Conditions Temperature C During operation 15 30 During down time 0 55 Humidity RH During operation 3...

Страница 13: ...y at least two individuals Hold the moving handles of the instrument from two sides to lift it out from the box Do not hold the plastic outer covering Do not hold the front panel Do not hold the door Caution Improper handling or movement of the AnaPrep 12 system will lead to instrument damages For proper and safe use of AnaPrep 12 install it in a location that is near an electrical outlet and has ...

Страница 14: ...ep 12 system 2 Connect Barcode Reader with the instrument 3 Remove the polystyrene foam block inside the instrument by following the steps below i Plug in the power cord to the instrument and connect to the electrical outlet ii Turn on the power switch iii Press the START button on the Control Pad After the piston module moves up remove the polystyrene foam block ...

Страница 15: ...MRevA Z1321001UB Active Date 02012016 Page 15 1 5 Overall View Front view with Sliding Door closed Front view with Sliding Door opened LCD Screen Sliding Door Moving Handle Operational Area UV Lamp Control Pad ...

Страница 16: ...nformation of operation instruction guide and program process status The Control Pad is used for selection of functions and input of programs and test codes LCD Screen Control Pad Number buttons Turns on off UV light Pause or terminate the program Start the program wake up from idle mode Confirmation Next Step Cancel Previous Step ...

Страница 17: ... any biohazardous operations Always follow standard safety procedures and wear the appropriate gloves mask and safety goggles when handling specimens and reagents Important Before starting extraction put on appropriate gloves mask and safety goggles When preparing samples for extraction be careful not to contaminate the samples with sweat saliva etc Power Switch Power Inlet ...

Страница 18: ...wer cable is connected securely to the AnaPrep 12 system 2 Connect the plug of the power cable to the outlet 2 2 Preparation The following preparations are required for the extraction operation Items to be prepared Gloves Mask Safety Goggles Reagent Cartridge s Reaction Chamber s Tip Holder Filtered tip ...

Страница 19: ... Reagent Kits 2 3 Extraction 1 Turn on the power switch then wait for the LCD screen to turn on and display AnaPrep 12 System Stand By 2 Press the Start button The system will initiate a self testing process initializing before entering steady mode Note The system will not respond to any information entered from the key pad until the completion of the self testing process ...

Страница 20: ...e the sample rack from the instrument 4 Load Reagent Cartridges and all plastic disposables Reaction Chamber Tip Holder Piercing Pin and Filtered Tip Insert the cartridges How to pull apart reagent cartridges Cut the foil at the dotted line with a finger nail then snap the cartridge apart ...

Страница 21: ...te 02012016 Page 21 Insert Reaction Chambers Caution Please make sure that all plastic accessories are loaded in the instrument before each run especially the Reaction Chambers otherwise this might cause serious damage to the instrument ...

Страница 22: ...F 753 3UMRevA Z1321001UB Active Date 02012016 Page 22 Insert Tip Holder showing only one row Insert Piercing Pins showing only one row ...

Страница 23: ...te 02012016 Page 23 Insert Filtered Tips showing only one row Note 1 Load one Reagent Cartridge and one set of plastic disposable per sample 2 Please note the positions of the piercing pin and filtered tip the second row should be EMPTY ...

Страница 24: ...cartridges are inserted into the Cartridge Tray tightly 1 12 cartridges may be loaded on the tray depending on the number of samples that you wish to process 5 Load Sample Tube and Elute Tube to Sample Rack on the bench sample tubes loaded in the S row marked on the rack elute tubes loaded in the the E row marked on the rack Insert Sample Tube on to Sample Rack Insert Elute Tube on to Sample Rack ...

Страница 25: ...me sample types before loading to Sample Tube Please refer to the handbooks of reagent kits for details Make sure the caps of Elute Tube are open as shown in the figure above 7 Place Sample Rack on the instrument platform Note Use two hands to handle the Sample Tray Make sure the Sample Tray is placed correctly in the instrument ...

Страница 26: ...volume will be shown on LCD screen after the protocol barcode is scanned 10 Follow the instructions displayed on the LCD screen to double check the operating steps before running the program 11 Press Enter to confirm The instrument will start to run the selected protocol program automatically until the whole process is completed Note It takes from 30 to 60 minutes to complete the extraction proces...

Страница 27: ...aste bin Do not re use the cartridges 16 If you re not using the instrument place the Sample Rack back in the instrument close the sliding door and push the Start button for 2 seconds to enter into sleep mode If not using the instrument for a longer period of time turn off the power switch 2 4 After Extraction Operation 1 Take out the Elute Tubes the extracted nucleic acids are stored in them Note...

Страница 28: ...ody outside 19 Cleaning of instrument body inside User User User After each use Bi weekly Bi weekly Preventive Service Engineer Annually Clean the Sample Tray with mild detergent and rinse with deionized water Allow the parts to dry before use Clean and disinfect the platform surface by wiping with deionized water followed by 70 ethanol Clean the instrument body by dusting gently with a dry soft c...

Страница 29: ...ack to the initial state Re load the cartridges or plastic wares according to the instructions shown in this manual Note The protocol will NOT resume after stopping and you may lose your samples Problem with motion sensors Turn off the power and remove all samples and plastic ware Contact your local representative Bubbles formed during extraction Missed adding sample or sample volume is lower than...

Страница 30: ... starting material Insufficient amount of magnetic beads added During shipping some magnetic bead solution may adhere to the sealing foil of the cartridge To collect any bead solution from the foil tap the cartridge to deposit the bead solution at the bottom of the well Clogged Tips resulting in DNA loss Ensure that the lysate does not contain particulates that could clog the end of the pipette ti...

Страница 31: ... sure the sample volume is at the recommended volume listed in the manual supplied with Reagent Kits Purified DNA repeatedly frozen and thawed Aliquot purified DNA and store at 4 C short term or 20 C long term Avoid repeated freezing and thawing DNA contaminated with DNases Maintain a sterile environment while working i e wear gloves and use DNase free reagents ...

Страница 32: ...ture 60 C to 70 C assuming the room temperature is 25 C Protocol Input Barcode Reader Built in Features LCD Display Screen Instrument Dimensions 47 cm W x 68 cm D x 51 cm H 18 5 in x 26 8 in x 20 1 in Weight 43 kg 94 8 lbs Input Power AC 100 240 V 240 VA 50 60 Hz Operating Temperature 15 30 C Operating Humidity 30 80 Fuse F3A 250V Temperatures allowed during transportation storage packaging 25 C t...

Страница 33: ...oduct ii Any part which fails to function properly under normal use provided that all warnings and cautions in this manual are observed will be repaired by the BioChain Institute Inc during the guarantee period free of charge iii If the problem is due to the causes listed below some charges may be applied for the repair even within the guarantee period Any failures due to improper use or that are ...

Страница 34: ... the AnaPrep 12 system When moving the AnaPrep 12 system to a new location perform the following tasks Warning Before carrying the AnaPrep 12 system disconnect the plug from the outlet If the power cable is damaged this may cause a device failure fire injury or electric shock Remove all attached parts Sample Rack Barcode Reader from the AnaPrep 12 system Important Note on Shipping AnaPrep 12 If th...

Страница 35: ...53 3UMRevA Z1321001UB Active Date 02012016 Page 35 BioChain Institute Inc www biochain com BioChain Institute Inc 39600 Eureka Dr Newark CA 94560 USA 1 510 783 8588 1 510 783 5386 techsupport biochain com ...

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