BIO RAD QX200, Инструкция по эксплуатации

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12 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 13
Chapter 3 Using QuantaSoft Software Chapter 3 Using QuantaSoft Software
4. Define Target 1 (channel 1, the FAM channel) and Target 2 (channel 2, the VIC or HEX channel). Assign
each assay a Name and sample Type .
Settings appear in the Applied Well Settings box as you enter them. When you are done, click Apply or OK
to save the information. The settings appear in the well in the plate map.
Tips:
Use the plate map to change the selected well without exiting the well editor.
To append sample or assay names with numbers incrementally through selected wells, select
the
Auto Inc check box next to Name .
Use standard Windows keyboard shortcuts for copying, pasting, and deleting selections (for
example, use
Ctrl+c to copy a selection, Ctrl+z to undo an operation, etc.).
3.1.2 Using the Experiment Editor
Use the experiment editor to define the experiment type. To open the experiment editor, select Experiment
> add experiment... in the well editor or select New or Edit (double-click on an experiment name) in the
Experiments window under Setup. Three types of experiments are possible: Absolute Quantification (ABS), Rare
Event Detection (RED), and Copy Number Variation (CNV). A default list of experiments is supplied at installation,
but you can create and save custom experiments; upgrade installations will preserve current experiment lists.
Settings are summarized in the Applied Well Settings box as you enter them. Click Apply or OK to save the
experiment information. The settings appear in the well in the plate map.
If the Sample Name, Experiment, Target Name, Target Type, and Supermix all match across multiple wells (all
are case sensitive), the software can identify these as “merged” and provide an option to view merged data
during analysis (see Section 3.3).
Fig. 10. Experiment editor. Options for a CNV experiment are shown.
Copy number options appear for CNV
experiments only
Customize the color codes for experiments (for
example, by experiment type, user, etc.)