AUTOMATED ELECTROPHORESIS
Experion
™
DNA 12K Analysis Kit
Quick Guide
1
2
3
4
5
Turn On Experion Electrophoresis Station and Launch Experion Software
Equilibrate Kit Reagents
I
Remove the DNA 12K stain, gel (or gel-stain solution, if available), and loading buffer from storage,
and equilibrate to room temperature
I
Invert each tube several times; vortex the contents and briefly centrifuge
Note: Protect stain and gel-stain solution from light at all times.
Prepare the Gel-Stain Solution
I
Transfer 200 µl DNA 12K gel (
green cap
) to a DNase-free 0.65 ml microcentrifuge tube
I
Add 10 µl DNA stain (
blue cap
) to the tube of 200 µl DNA 12K gel; vortex and briefly centrifuge
I
Transfer gel-stain solution to a spin filter tube and centrifuge at 1,500 x g for 10 min
Note: Filtered gel-stain stored at 4°C may be used for up to 1 month. Discard after 1 month; do not refilter.
Prepare the DNA Samples and DNA 12K Ladder
I
Remove the DNA 12K ladder (
clear cap
) from storage, briefly centrifuge, and then vortex to mix;
place the tube of ladder on ice
I
Prepare dilutions of DNA sample in TE buffer or DNase-free water to concentrations within the
linear range of this assay (0.5–50 ng/µl total DNA); make sure the salt concentration of the sample
does not exceed 250 mM KCl (or 250 mM NaCl), 15 mM MgCl
2
Prime the Chip
I
Remove a DNA chip from its packaging and place it on the chip
platform in the Experion priming station
I
Add 9 µl gel-stain solution into the gel priming well
I
Close the lid of the priming station, set the pressure to
C
,
and set the time to
1
I
Press the
Start
button
I
When priming is complete, remove the primed chip from the
priming station
I
Flip the chip over, and visually inspect the microchannels for
trapped air bubbles or incomplete priming
Note: Do
not
touch the glass surface of the chip.
DNA
1K = C3
12K = C1
Gel
priming
well
