Binder APT.line C 150 Скачать руководство пользователя

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C 150 (E2) 06/2011

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2.2

Unit overview

Figure 7: CO

incubator C 150 (door hinged right)

(1)

Triangle instrument panel with RP1 controller for temperature and CO

and main power switch (2)

(3)

Gas mixing head CO

(4) CO

sensor

(5)

Pt 100 temperature sensor

(6) Shelves

(7)

Lower housing cover

(8)

Silicone measuring port

(9)

Inner glass door

(10) Water

pan

(3)

(4)

(5)

(6)

(7)

(1)

(8)

(9)

(10)

Содержание APT.line C 150

Страница 1: ...0080 9140 0080 right 100 V C150 9040 0083 9140 0083 left 100 V BINDER GmbH Address Post office box 102 D 78502 Tuttlingen Tel 49 7462 2005 0 Fax 49 7462 2005 100 Internet http www binder world com E...

Страница 2: ...laments und des Rates vom 15 Dezember 2004 zur Angleichung der Rechtsvorschriften der Mitglied staaten ber die elektromagnetische Vertr glichkeit und zur Aufhebung der Richtlinie 89 336 EWG Directive...

Страница 3: ...commande et de laboratoire Exigences relatives la CEM Partie 1 Exigences g n rales CEI 61326 1 2005 AC1 2008 NF EN 61326 1 2006 mod EN 61326 2 2 2006 Elektrische Mess Steuer Regel und Laborger te EMV...

Страница 4: ...f delivery 17 3 2 Guidelines for safe lifting and transportation 18 3 3 Storage 19 3 4 Location of installation and ambient conditions 19 4 INSTALLATION AND CONNECTIONS 20 4 1 Shelves 20 4 2 CO2 senso...

Страница 5: ...12 1 2Measuring CO2 directly via chemical indicator tubes 51 12 1 3Measuring CO2 directly with an electronic infrared measuring device 52 12 2 Temperature reference measurement 52 13 OPTIONS 52 13 1 S...

Страница 6: ...al Republic of Germany 67 17 4 Disposal of the unit in the member states of the EC except for the Federal Republic of Germany 68 17 5 Disposal of the unit in non member states of the EC 69 18 TECHNICA...

Страница 7: ...ites for hazard free use and safety during operation and maintenance In no event shall BINDER be held liable for any dam ages direct or incidental arising out of or related to the use of this manual T...

Страница 8: ...duct and or its functions or to property in its proximity 1 2 2 Safety alert symbol Use of the safety alert symbol indicates a risk of injury Observe all measures that are marked with the safety alert...

Страница 9: ...he hazard mandatory action Observe all other notes and information not necessarily emphasized in the same way in order to avoid disruptions that could result in direct or indirect injury or property d...

Страница 10: ...50 UL door hinged right Figure 4 Position of labels on the CO2 incubator C 150 UL door hinged left Keep safety labels complete and legible Replace safety labels that are no longer legible Contact BIND...

Страница 11: ...arking Electrical and electronic equipment manufactured placed on the market in the EC after 13 August 2005 and to be disposed of in a separate collection according to directive 2002 96 EC on waste el...

Страница 12: ...er of death Do NOT operate the unit in potentially explosive areas KEEP explosive dust or air solvent mixtures AWAY from the unit The CO2 incubator C 150 does not dispose of any measures of explosion...

Страница 13: ...nded use Series C 150 incubators are suitable for the cultivation of mammal cells under typical conditions of approx 37 C 98 6 F The incubator permits setting defined pH conditions by common NaHCO3 bu...

Страница 14: ...ng system in combination with the permanent mixture of CO2 gas through a special proprietary gas mixing head developed by BINDER allows precise and con stant CO2 concentrations for long periods This c...

Страница 15: ...hinged right 1 Triangle instrument panel with RP1 controller for temperature and CO2 and main power switch 2 3 Gas mixing head CO2 4 CO2 sensor 5 Pt 100 temperature sensor 6 Shelves 7 Lower housing co...

Страница 16: ...e 13 Connection socket for zero voltage relay alarm contact 14 Quick acting closure socket for CO2 gas bottle 15 Ethernet interface for computer communication option 2 4 Triangle instrument panel C 15...

Страница 17: ...facturer may have caused traces of the shelves on the inner surfaces This has no impact on the function and performance of the unit Please remove any transportation protection devices and adhesives in...

Страница 18: ...riginal packaging only Secure the CO2 incubator with transport straps for transport Do NOT lift or transport the unit using the door handle the door or the lower housing Lift the unit at the four lowe...

Страница 19: ...id contamination never place the unit directly on the floor CAUTION Danger of overheating Damage to the unit Do NOT set up units in non ventilated recesses Ensure sufficient ventilation for dispersal...

Страница 20: ...al pressure CO2 lost during 12h at 5 vol without door opening approx 2 g i e 0 001 cubic meters 0 035 cubic feet under normal pressure 1013 mbar 14 7 psi An example of how to evaluate laboratory volum...

Страница 21: ...sor Do NOT expose the CO2 sensor to hot air sterilization The CO2 sensor head was especially adjusted for the specific chamber To avoid confusion an adhesive label with a serial number is adhered to t...

Страница 22: ...ives is excluded from the liability agreement If required you can add microbiologically inhibiting substances such as copper chips copper sulphate or ethylene diamine tetra vinegar acid EDTA in a conc...

Страница 23: ...a hose connection to the incubator The recovery times of the gas concentrations inside the chamber after opening the door are indicated in the technical data chap 18 4 and refer to a connection press...

Страница 24: ...hose nozzle off the quick acting closure socket 4 4 3 Gas bottle connection kit option The following parts for connecting one bottle with the input connectors of the incubator are included Pressure r...

Страница 25: ...d refer to a connection pressure of 2 0 bar 29 psi Decreasing supply pressure will result in longer recovery times 4 5 Electrical connection The CO2 incubator C 150 has a fixed power supply connection...

Страница 26: ...day and in a well ventilated location 6 Function overview of the RP1 controller The RP1 controller controls the following values inside the CO2 incubator Temperature in C Range 7 C 12 6 F above ambien...

Страница 27: ...m 1 0 vol Delay time tolerance range alarm CO2 after opening the door 15 min Password to lock unlock the HAND operating functions 0 Password to access operating mode USER 1 6 2 Performance after turni...

Страница 28: ...bration time is approx 2 hours CO2 After approx 5 minutes the CO2 concentration equilibrates automatically to the preset value If there is no accordance between the actual and set value shown in the d...

Страница 29: ...function i e the C 150 will from now on regulate at the new value If you do not want to accept the modification of a value wait 30 seconds without pressing any button The controller reverts to Normal...

Страница 30: ...en exceeded the safety controller triggers an alarm For technical reasons the offset value should be at least 2 C factory setting 0 to 999 Max 3 digit numeric password for locking unlocking the operat...

Страница 31: ...l the value OP U operating mode USER appears in the upper display Press MODE The display to enter the password appears The operating mode USER is password protected by a number The password is preset...

Страница 32: ...99 min Temperature alarm delay time Period until the main controller triggers a tolerance range alarm after closing the door This prevents alarms constantly being triggered during the unstable operati...

Страница 33: ...t required to operate the C 150 Its value should always be zero Set point type for safety controller Limit Lit In operating modes USER and HAND you can define the limit value Al S Set point type for s...

Страница 34: ...djustment serves to adjust the temperature sensor OFF or 10 0 vol to 110 0 vol CO2 final value adjustment serves to adjust the CO2 sensor The operating function At S specifies the safety controller se...

Страница 35: ...n the upper display OP L operating mode LOCK is now displayed It is visible only if a password was set in operating mode HAND via operating function PA H Confirm the selected operating mode with the M...

Страница 36: ...all controller functions are taken out of operation The gas inlet valves are closed so that no gas can escape into the ambient air The zero voltage relay alarm output 13 chap 10 3 is switched to alarm...

Страница 37: ...R with the arrow keys Password request Password entry factory setting 1 4 x Entry of altitude Entry of the current altitude km above sea level with arrow keys After 30 seconds the controller reverts t...

Страница 38: ...oint Entry of CO2 set point with arrow keys After 30 seconds the controller reverts to Normal Display automatically When setting a lower temperature set point in order to save time we recommend coolin...

Страница 39: ...s safety controller activity The LED ALARM flashes the LED is lit At the same time there is an additional audible alert buzzer Pressing the EXIT button turns off the audible alarm When the temperature...

Страница 40: ...ER Operating mode HAND Operating mode USER If operating mode HAND appears toggle to operating mode USER with the arrow keys Password request Password entry factory setting 1 12 x Selecting the set poi...

Страница 41: ...AND and USER You can set a limit value With set point type Offset OFS operating function Ot S safety controller set point as offset value is displayed in operating modes HAND and USER You can set an o...

Страница 42: ...p 9 2 1 a different display appears Entry of limit value or offset value With set point type Limit Enter a limit value Entry of limit value using the arrow keys or With set point type Offset Enter an...

Страница 43: ...sensor for interior heating CO2 sensor not connected If more than one alarm signal is sent simultaneously they are displayed in a cycle except for alarms 995 to 999 These take priority over all other...

Страница 44: ...nd connection socket Do NOT exceed the maximum switching load of 24V AC DC 2 0 Amp Do NOT connect any devices with a higher loading capacity The alarm message on the controller display remains display...

Страница 45: ...ure sufficient ventilation around the installation location to prevent any buildup of heat in the unit Check whether the sterilization cycle was aborted and the unit has returned to its standard opera...

Страница 46: ...contact BINDER Service To decrease the temperature proceed as follows Turn off the unit Open both unit doors for approx 5 minutes Turn on the unit again You can restart normal operation as soon as th...

Страница 47: ...heck that the outer door is closed properly Check the door s seals for any damage Replace any damaged seals If the above points do not reveal the source of the fault it may be that the unit is faulty...

Страница 48: ...sure reducer of each bottle The recovery times of the gas concentrations inside the chamber following the door being opened which are indicated in the technical data chap 18 4 refer to a connection pr...

Страница 49: ...BINDER Service 11 Error messages While the controller is operating or when you turn it on or change the operation mode fault signals may be emitted that are caused by the controller malfunctioning int...

Страница 50: ...d base equilibrium of the buffer system in the culture media NaHCO3 buffers the common media From the pH value of the medium it is possible to conclude its CO2 concentration Figure 27 shows the relati...

Страница 51: ...of the inner chamber volume by pressing the pump fully together and releasing it afterwards 5 The standardized volume is suctioned through the glass tube and the chemical indicator changes its color b...

Страница 52: ...vice it is important to use a device traceable to an acknowledged standards calibration institu tion DKD PTB for Germany with valid calibration certificate Note The cable of the sensor must be thin en...

Страница 53: ...acking adapter for direct thermal de coupled stacking of two incubators The stacking adapter ensures the exact maintenance of the set incubation parameters also during sterili zation of the other unit...

Страница 54: ...ing fea tures and the lab personnel Keep pipettes and instruments sterile after autoclaving Bio safety cabinets laminar air flow should have a minimum of items apart from aspirator tube and burner Ite...

Страница 55: ...ass door are glued from the outside which also aids cleaning of the inner chamber Removable parts for cleaning and autoclaving The shelves are easily removed without screws It is possible to autoclave...

Страница 56: ...light If the temperature distribution inside the chamber becomes uneven condensation may form on the cooler surfaces Maintain distances from the wall rear 100 mm 3 94 in sides 50 mm 1 97 in Do not pla...

Страница 57: ...sing the neutral cleaning agent Art No 1002 0016 CO2 sensor Alcohol based solutions Do not immerse the CO2 sensor into the solution Disinfection with alcohol or an alcohol based surface disinfectant w...

Страница 58: ...y dry and ventilated as explosive gases may form during the sterilization process 2 You can sterilize the shelves in a sterilizer or autoclave With every decontamination disinfection method always use...

Страница 59: ...the CO2 sensor during operation Damage to the CO2 sensor Connect or remove the CO2 sensor only with the unit turned off Recommended procedure 1 Turn off the unit 2 Pull out the sensor 3 Spray the sens...

Страница 60: ...e to the CO2 sensor Do NOT expose the CO2 sensor to hot air sterilization The CO2 sensor head was especially adjusted for your specific chamber To avoid confusion an adhe sive label with a serial numb...

Страница 61: ...ode HAND is locked This is displayed by an error message If this happens unlock the operating mode HAND chap 7 4 Figure 33 Error message if operating mode HAND is lo cked or if the CO2 sensor is still...

Страница 62: ...mum temperature of 60 C 140 F Turn on the unit chap 5 The unit is now ready to operate 15 5 Aborting the hot air sterilization If the sterilization procedure is aborted prematurely whether effective s...

Страница 63: ...When the inner chamber has cooled down to a value below 60 C 140 F turn off the unit and plug in the CO2 sensor The CO2 sensor is temperature resistant up to a maximum temperature of 60 C 140 F Turn o...

Страница 64: ...mperature alarm the C 150 will trigger alarm signals You can ignore this alarm and turn off the buzzer of the safety controller by pressing EXIT If necessary repeat the sterilization When the inner ch...

Страница 65: ...hotline Asia Pacific 852 39070500 or 852 39070503 BINDER service hotline Russia and CIS 7 495 98815 17 BINDER Internet website http www binder world com BINDER address BINDER GmbH post office box 102...

Страница 66: ...ete address contact person and availability of that person Exact location of the BINDER product in your facility A contamination clearance certificate chap 19 must be faxed in advance The authorizatio...

Страница 67: ...indications for appropriate storage chap 3 3 Final decommissioning Dispose of the unit as described in chap 17 3 to 17 5 When restarting the unit please pay attention to the corresponding information...

Страница 68: ...tion clearance certificate chap 19 and enclose it with the unit WARNING Contamination of the device with toxic infectious or radioactive substances Danger of intoxication Danger of infection NEVER tak...

Страница 69: ...rom the unit Prior to disposal disinfect the unit from all sources of infection Be aware that sources of infection may also be located outside the inner chamber If you cannot safely remove all sources...

Страница 70: ...y bearing a valid calibration certificate During factory calibration and adjustment test gases with an analyzed concentration and with adapted flow quantity serve to calibrate the sensor system for CO...

Страница 71: ...erature data Temperature range 7 C 12 6 F above ambient up to C F 50 122 Temperature fluctuation K 0 1 Temperature uniformity variation at 37 C 98 6 F K 0 4 Recovery time 1 after door open for 30 sec...

Страница 72: ...es 2 Use this value for sizing air condition systems All technical data is specified for units with standard equipment at an ambient temperature of 25 C 77 F and a power supply voltage fluctuation of...

Страница 73: ...auto diagnosis system with zero voltage relay output Temperature safety device class 3 1 acc to DIN 12880 Lockable door Tightly closing inner glass door 3 perforated shelves stainless steel 1 4301 V2...

Страница 74: ...03 Manual for primary human cell culture 7001 0079 Petri dish incubation rack rack for 6 x 6 Petri dishes stainless steel 6006 0167 Petri dish incubation rack rack for 6 Petri dishes stainless steel 6...

Страница 75: ...C 150 E2 06 2011 page 75 78 18 9 Dimensions C 150...

Страница 76: ...t Bauteil eintrifft Eine weitere Ko pie soll dem Ger t Bauteil beigef gt sein Ggf ist auch die Spedition zu informieren Incomplete information or non conformity with this procedure will inevitably lea...

Страница 77: ...substances weder giftige noch sonstige gef hrliche Stoffe enth lt oder solche anhaften That eventually generated reaction products are non toxic and also do not represent a hazard auch evtl entstande...

Страница 78: ...en entsteht haften und BINDER gegen eventuell entstehende Schadenanspr che Dritter freistellen We are aware that in accordance with Article 823 of the German Civil Code BGB we are directly liable with...

Страница 79: ......

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