BD FACSymphony A3, Руководство пользователя

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User’s Guide 64
Cytometer settings workflow
Introduction This topic describes how to optimize cytometer settings. The
optimization is performed using the Cytometer Setup and
Tracking, Application Settings, and Compensation Setup features
of BD FACSDiva software.
When to optimize
settings Before you record data for a sample, optimize the cytometer
settings for the sample type and fluorochromes used.
Manual
compensation Compensation setup automatically calculates compensation
settings. If you choose to perform compensation manually, not all
of the following instructions apply. For detailed instructions, see
the
BD FACSDiva Software Reference Manual .
First-time users
If you are performing the procedures in this workflow for the first
time, you should be familiar with BD FACSDiva software
concepts: workspace components, cytometer and acquisition
controls, and tools for data analysis.
For additional details, see the BD FACSDiva Software Reference
Manual.
Before you begin Start the cytometer and perform the setup and QC procedures. See
Cytometer setup (page 33) .