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User’s Guide
102
Cytometer troubleshooting
Introduction
This topic describes possible problems and recommended solutions
for BD FACSymphony A3 and BD FACSymphony A5 flow
cytometer issues.
Droplets are visible
on the SIP
Possible causes
Recommended solutions
Worn O-ring in the retainer
Replace the O-ring. See
Outer sleeve is not seated in
the retainer
1. Loosen the retainer.
2. Push the outer sleeve up into the
retainer until seated.
3. Tighten the retainer.
Outer sleeve is not on the
sample injection tube
Replace the outer sleeve.
1. Loosen the retainer.
2. Slide the outer sleeve over the
sample injection tube until it is
seated.
3. Tighten the retainer.
Waste line is pinched,
preventing proper aspiration
Check the waste line.
Waste tank is full
Empty the waste tank.
Droplet containment vacuum
is not functioning
Call your BD service representative.
The mode is not set to HTS.
Change the mode to HTS by pressing
down the Mode button for more than
3 seconds.
Содержание FACSymphony A3
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Страница 97: ...Chapter 6 Recording and analyzing data 97 e Click OK 12 Print the analysis...
Страница 98: ...User s Guide 98 Your global worksheet analysis objects should look like the following...
Страница 120: ...User s Guide 120 14 Adjust the red laser area scaling factor until the APC A signal matches the APC H signal if needed...